Base editing: a brief review and a practical example

Choe Dongwook C.; Musunuru Kiran

doi:10.7555/JBR.34.20200003

Volume 35 Issue 2

Mar. 2021

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Choe Dongwook C., Musunuru Kiran. Base editing: a brief review and a practical example[J]. The Journal of Biomedical Research, 2021, 35(2): 107-114. doi: 10.7555/JBR.34.20200003

Citation:

Choe Dongwook C., Musunuru Kiran. Base editing: a brief review and a practical example[J]. The Journal of Biomedical Research, 2021, 35(2): 107-114. doi: 10.7555/JBR.34.20200003

Choe Dongwook C., Musunuru Kiran. Base editing: a brief review and a practical example[J]. The Journal of Biomedical Research, 2021, 35(2): 107-114. doi: 10.7555/JBR.34.20200003

Citation:

Choe Dongwook C., Musunuru Kiran. Base editing: a brief review and a practical example[J]. The Journal of Biomedical Research, 2021, 35(2): 107-114. doi: 10.7555/JBR.34.20200003

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Base editing: a brief review and a practical example

doi: 10.7555/JBR.34.20200003

Division of Cardiology and Cardiovascular Institute, Department of Medicine, Department of Genetics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA

More Information

Corresponding author: Kiran Musunuru, Perelman School of Medicine at the University of Pennsylvania, 3400 Civic Center Boulevard, Building 421, 11-104 Smilow Center for Translational Research, Philadelphia, PA 19104, USA. Tel/Fax: +1-215-573-4717/+1-215-746-7415, E-mail: kiranmusunuru@gmail.com
Received: 2020-01-02
Revised: 2020-04-29
Accepted: 2020-06-10

Published: 2020-07-31

Issue Date: 2021-03-26

Abstract

Abstract

Genome editing has undergone rapid development in recent years, yielding new approaches to make precise changes in genes. In this review, we discuss the development of various adenine and cytosine base-editing technologies, which share the ability to make specific base changes at specific sites in the genome. We also describe multiple applications of base editing in vitro and in vivo. Finally, as a practical example, we demonstrate the use of a cytosine base editor and an adenine base editor in human cells to introduce and then correct a prevalent mutation responsible for hereditary tyrosinemia type 1.
- base editing,
- CRISPR,
- genetics,
- genome editing,
- mutation

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References(32)

References

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