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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 38 Issue 1
Jan.  2024
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Yejin Yao, Baolu Shi, Xiangzheng Zhang, Xin Wang, Shuangyue Li, Ying Yao, Yueshuai Guo, Dingdong Chen, Bing Wang, Yan Yuan, Jiahao Sha, Xuejiang Guo. Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis[J]. The Journal of Biomedical Research, 2024, 38(1): 24-36. DOI: 10.7555/JBR.37.20230055
Citation: Yejin Yao, Baolu Shi, Xiangzheng Zhang, Xin Wang, Shuangyue Li, Ying Yao, Yueshuai Guo, Dingdong Chen, Bing Wang, Yan Yuan, Jiahao Sha, Xuejiang Guo. Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis[J]. The Journal of Biomedical Research, 2024, 38(1): 24-36. DOI: 10.7555/JBR.37.20230055
shu

Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis

  • 1.

    State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China

  • 2.

    Reproductive and Genetic Branch, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China

  • 3.

    State Key Laboratory of Reproductive Medicine and Offspring Health, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, Jiangsu 211166, China

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  • Corresponding author:

    Jiahao Sha, State Key Laboratory of Reproductive Medicine and Offspring Health, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu 211166, China. E-mail: shajh@njmu.edu.cn

    Xuejiang Guo, State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu 211166, China. E-mail: guo_xuejiang@njmu.edu.cn

  • △These authors contributed equally to this work.

  • Received Date: March 16, 2023
  • Revised Date: May 28, 2023
  • Accepted Date: May 28, 2023
  • Available Online: June 02, 2023
  • Published Date: December 07, 2023
    Graphical Abstract
    • Abstract

  • Peroxisomes are organelles enclosed by a single membrane and are present in various species. The abruption of peroxisomes is correlated with peroxisome biogenesis disorders and single peroxisomal enzyme deficiencies that induce diverse diseases in different organs. However, little is known about the protein compositions and corresponding roles of heterogeneous peroxisomes in various organs. Through transcriptomic and proteomic analyses, we observed heterogenous peroxisomal components among different organs, as well as between testicular somatic cells and different developmental stages of germ cells. As Pex3 is expressed in both germ cells and Sertoli cells, we generated Pex3 germ cell- and Sertoli cell-specific knockout mice. While Pex3 deletion in Sertoli cells did not affect spermatogenesis, the deletion in germ cells resulted in male sterility, manifested as the destruction of intercellular bridges between spermatids and the formation of multinucleated giant cells. Proteomic analysis of the Pex3-deleted spermatids revealed defective expressions of peroxisomal proteins and spermiogenesis-related proteins. These findings provide new insights that PEX3-dependent peroxisomes are essential for germ cells undergoing spermiogenesis, but not for Sertoli cells.

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  • The current study was supported by grants from the National Natural Science Foundation of China (Grant No. 31890784 to J.S.), National Key R&D Program (Grant No. 2021YFC2700200 to X.G.), National Natural Science Foundation of China (Grant Nos. 92068109 and 82122025 to Yan Yuan), Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Grant No. 21KJA310007 to Yan Yuan), and Science Foundation of Gusu School (Grant No. GSKY20220101 to J.S.).

    We thank Jinyang Cai for assistance in microscopy and Zibin Wang for ultrastructure analysis from Nanjing Medical University.

    CLC number: R321.1, Document code: A

    The authors reported no conflict of interests.

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