4.6

CiteScore

2.2

Impact Factor
  • ISSN 1674-8301
  • CN 32-1810/R
Volume 26 Issue 1
Dec.  2011
Turn off MathJax
Article Contents
Abstract
Related Articles
Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. DOI: 10.1016/S1674-8301(12)60002-5
Citation: Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. DOI: 10.1016/S1674-8301(12)60002-5
shu

Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

  • 1.

    Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya/Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

  • 2.

    Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya

  • 3.

    Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

Funds: 

This work was supported by a grant from the National Council for Science and Technology, Government of Kenya (No. NCST 51003 CALL2 226)

More Information
  • Received Date: July 22, 2011
    Graphical Abstract
    • Abstract
  • In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.
    • FullText(HTML)
    • References (0)
    • Related Articles

  • Related Articles

    [1]Gege Yuan, Jiachen Wang, Shuangshuang Qiu, Yunfei Zhu, Qing Cheng, Laihua Li, Jiahao Sha, Xiaoyu Yang, Yan Yuan. Title: Improving in vitro induction efficiency of human primordial germ cell-like cells using N2B27 or NAC-based medium[J]. The Journal of Biomedical Research. DOI: 10.7555/JBR.38.20240433
    [2]Tiwari-Heckler Shilpa, Jiang Z. Gordon, Popov Yury, J. Mukamal Kenneth. Daily high-dose aspirin does not lower APRI in the Aspirin-Myocardial Infarction Study[J]. The Journal of Biomedical Research, 2020, 34(2): 139-142. DOI: 10.7555/JBR.33.20190041
    [3]Shen Tian, Han Bo'ang, Leng Yan, Yan Sen, Shi Junfeng, Yue Shen, Cheng Steven Y. Sonic Hedgehog stimulates migration of MCF-7 breast cancer cells through Rac1[J]. The Journal of Biomedical Research, 2019, 33(5): 297-307. DOI: 10.7555/JBR.32.20180100
    [4]Huan Liu, Shijiang Zhang, Yongfeng Shao, Xiaohu Lu, Weidong Gu, Buqing Ni, Qun Gu, Junjie Du. Biomechanical characterization of a novel ring connector for sutureless aortic anastomosis[J]. The Journal of Biomedical Research, 2018, 32(6): 454-460. DOI: 10.7555/JBR.31.20170011
    [5]Minbo Zang, Qiao Zhou, Yunfei Zhu, Mingxi Liu, Zuomin Zhou. Effects of chemotherapeutic agent bendamustine for nonhodgkin lymphoma on spermatogenesis in mice[J]. The Journal of Biomedical Research, 2018, 32(6): 442-453. DOI: 10.7555/JBR.31.20170023
    [6]Huanqiang Wang, Congying Yang, Siyuan Wang, Tian Wang, Jingling Han, Kai Wei, Fucun Liu, Jida Xu, Xianzhen Peng, Jianming Wang. Cell-free plasma hypermethylated CASZ1, CDH13 and ING2 are promising biomarkers of esophageal cancer[J]. The Journal of Biomedical Research, 2018, 32(6): 424-433. DOI: 10.7555/JBR.32.20170065
    [7]Fengzhen Wang, Mingwan Zhang, Dongsheng Zhang, Yuan Huang, Li Chen, Sunmin Jiang, Kun Shi, Rui Li. Preparation, optimization, and characterization of chitosancoated solid lipid nanoparticles for ocular drug delivery[J]. The Journal of Biomedical Research, 2018, 32(6): 411-423. DOI: 10.7555/JBR.32.20160170
    [8]Sukhbir Kaur, Tejinder Kaur, Jyoti Joshi. Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice[J]. The Journal of Biomedical Research, 2016, 30(4): 304-313. DOI: 10.7555/JBR.30.20150125
    [9]Samuel W. Mwololo, Joshua M. Mutiso, John C. Macharia, Alain J. Bourdichon, Michael M. Gicheru. In vitro activity and in vivo efficacy of a combination therapy of diminazene and chloroquine against murine visceral leishmaniasis[J]. The Journal of Biomedical Research, 2015, 29(3): 214-223. DOI: 10.7555/JBR.29.20140072
    [10]Joshua M. Mutiso, John C. Macharia, Michael M. Gicheru. A review of adjuvants for Leishmania vaccine candidates[J]. The Journal of Biomedical Research, 2010, 24(1): 16-25.

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (7137) PDF downloads (175) Cited by()
    Related

    ©2023 by the Editorial Department of the Journals of Nanjing Medical University. All rights reserved.   苏ICP备05071376号-5

    Supported by: Beijing Renhe Information Technology Co., Ltd. E-mail: info@rhhz.net  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return