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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 26 Issue 1
Dec.  2011
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Abstract
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Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. DOI: 10.1016/S1674-8301(12)60002-5
Citation: Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. DOI: 10.1016/S1674-8301(12)60002-5
shu

Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

  • 1.

    Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya/Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

  • 2.

    Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya

  • 3.

    Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

Funds: 

This work was supported by a grant from the National Council for Science and Technology, Government of Kenya (No. NCST 51003 CALL2 226)

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  • Received Date: July 22, 2011
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    • Abstract
  • In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.
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