• ISSN 1674-8301
  • CN 32-1810/R
Volume 30 Issue 4
Jul.  2016
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Sukhbir Kaur, Tejinder Kaur, Jyoti Joshi. Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice[J]. The Journal of Biomedical Research, 2016, 30(4): 304-313. doi: 10.7555/JBR.30.20150125
Citation: Sukhbir Kaur, Tejinder Kaur, Jyoti Joshi. Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice[J]. The Journal of Biomedical Research, 2016, 30(4): 304-313. doi: 10.7555/JBR.30.20150125

Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice

doi: 10.7555/JBR.30.20150125
Funds:

the Indian Council of Medical Research, Department of Health Research, India

  • Received: 2015-09-01
  • Revised: 2015-11-05
  • Issue Date: 2016-07-28
  • The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis. Inbred BALB/c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1(+) encoding T cell epitopes of gp63 and Hsp70 individually and in combination.Animals were challenged intracardially with 107 promastigotes of Leishmania donovani 10 days post immunization and sacrificed 1, 2 and 3 months post challenge. The immunized animals revealed a significant reduction (P < 0.05)in splenic and hepatic parasite burden as compared to the infected controls. Maximum reduction in parasite load (P < 0.05) was observed in animals treated with a combination of pcDNA/gp63 and pcDNA/Hsp70. These animals also showed heightened DTH response, increased IgG2a, elevated Th1 cytokines (IFN-γ and IL-2) and reduced IgG1 and IL-10 levels. Thus, mice immunized with the cocktail vaccine exhibited significantly greater protection in comparison to those immunized with individual antigens.

     

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