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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 36 Issue 2
Mar.  2022
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Cui Qi, Jiaqi Zhang, Yuanyuan Wang, Mingyan Lin, Jun Gao, Haiying Lu. Valproic acid enhances neurosphere formation in cultured rat embryonic cortical cells through TGFβ1 signaling[J]. The Journal of Biomedical Research, 2022, 36(2): 127-140. DOI: 10.7555/JBR.36.20210109
Citation: Cui Qi, Jiaqi Zhang, Yuanyuan Wang, Mingyan Lin, Jun Gao, Haiying Lu. Valproic acid enhances neurosphere formation in cultured rat embryonic cortical cells through TGFβ1 signaling[J]. The Journal of Biomedical Research, 2022, 36(2): 127-140. DOI: 10.7555/JBR.36.20210109
shu

Valproic acid enhances neurosphere formation in cultured rat embryonic cortical cells through TGFβ1 signaling

  • 1.

    Department of Neurobiology, Key Laboratory of Human Functional Genomics of Jiangsu, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, China

  • 2.

    Department of Rehabilitation Medicine, Jiangsu Shengze Hospital affiliated to Nanjing Medical University, Suzhou, Jiangsu 215228,China

  • 3.

    Department of Neurology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, China

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  • Corresponding author:

    Jun Gao, Department of Neurobiology, School of Basic Medical Sciences, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, Jiangsu 211166, China. Tel: +86-25-86869347, E-mail: gaojun@njmu.edu.cn

    Haiying Lu, Department of Neurology, Children's Hospital of Nanjing Medical University, 72 Guangzhou Road, GulouDistrict, Nanjing, Jiangsu 210008, China. Tel: +86-25-83116827, E-mail: hylunj@163.com

  • Received Date: July 15, 2021
  • Revised Date: November 29, 2021
  • Accepted Date: December 06, 2021
  • Available Online: February 14, 2022
    Graphical Abstract
    • Abstract
  • This study aimed to investigate the effect and mechanism of valproic acid (VPA) on the neurosphere formation in rat embryonic cortical cells. We used free-floating neurosphere formation as a model system to evaluate the VPA on the proliferation of neural stem cells (NSCs). We found a time- and dose-dependent increase in neurosphere formation and NSC proliferation after VPA treatment. Further RNA-seq analysis demonstrated that the upregulated TGFβ1 signaling might attribute to the effect of VPA on the neurosphere formation and NSC proliferation. Consistently, the neurosphere formation and NSC proliferation were blocked by the treatment with SB431542, an inhibitor of TGFβ1 receptor. Moreover, in a coculture system, NSCs treated with VPA significantly reduced the oxygen-glucose deprivation-induced neuronal apoptosis. Taken together, our results showed that VPA could enhance neurosphere formation and NSC proliferation by activating TGFβ1, which might be a novel therapeutic strategy for neurological disorders.
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    • References (28)
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