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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 36 Issue 5
Sep.  2022
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Qian Sun, Yusi Hu, Saiyue Deng, Yanyu Xiong, Zhili Huang. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging[J]. The Journal of Biomedical Research, 2022, 36(5): 358-367. DOI: 10.7555/JBR.36.20220099
Citation: Qian Sun, Yusi Hu, Saiyue Deng, Yanyu Xiong, Zhili Huang. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging[J]. The Journal of Biomedical Research, 2022, 36(5): 358-367. DOI: 10.7555/JBR.36.20220099
shu

A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging

  • 1.

    Department of Pharmacology, School of Basic Medical Science, Fudan University, Shanghai 200032, China

  • 2.

    State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China

  • 3.

    Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China

Funds: This study was supported in part by Shanghai Committee of Science and Technology (Grant No. 20ZR1403500) and the Shanghai Medical Research Council. The cartoon image was obtained from Scidraw.io.
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  • Corresponding author:

    Zhili Huang, Department of Pharmacology, School of Basic Medical Science, Fudan University, 130 Dong'an Road, Shanghai 200032, China. Tel/Fax: +86-21-54237043/+86-21-54237103, E-mail: huangzl@fudan.edu.cn

  • Received Date: April 30, 2022
  • Revised Date: June 18, 2022
  • Accepted Date: July 10, 2022
  • Available Online: August 09, 2022
    Graphical Abstract
    • Abstract
  • Astrocytes, the multi-functional glial cells with the most abundant population in the brain, integrate information across their territories to regulate neuronal synaptic and cerebrovascular activities. Astrocytic calcium (Ca2+) signaling is the major readout of cellular functional state of astrocytes. The conventional two-photon in vivo imaging usually focuses on a single horizontal focal plane to capture the astrocytic Ca2+ signals, which leaves >80% spatial information undetected. To fully probe the Ca2+ activity across the whole astrocytic territory, we developed a pipeline for imaging and visualizing volumetric astrocytic Ca2+ time-lapse images. With the pipeline, we discovered a new signal distribution pattern from three-dimensional (3D) astrocytic Ca2+ imaging data of mice under isoflurane anesthetic states. The tools developed in this study enable a better understanding of the spatiotemporal patterns of astrocytic activity in 3D space.
    • FullText(HTML)

  • This study was supported in part by Shanghai Committee of Science and Technology (Grant No. 20ZR1403500) and the Shanghai Medical Research Council. The cartoon image was obtained from Scidraw.io.

    CLC number: R338.2, Document code: A

    The authors reported no conflict of interests.

    △These authors contributed equally to this work.

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