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Volume 35 Issue 2
Mar.  2021
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Channabasavaiah B. Gurumurthy, Thomas L. Saunders, Masato Ohtsuka. Designing and generating a mouse model: frequently asked questions[J]. The Journal of Biomedical Research, 2021, 35(2): 76-90. doi: 10.7555/JBR.35.20200197
Citation: Channabasavaiah B. Gurumurthy, Thomas L. Saunders, Masato Ohtsuka. Designing and generating a mouse model: frequently asked questions[J]. The Journal of Biomedical Research, 2021, 35(2): 76-90. doi: 10.7555/JBR.35.20200197

Designing and generating a mouse model: frequently asked questions

doi: 10.7555/JBR.35.20200197
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  • Corresponding author: Channabasavaiah B. Gurumurthy, Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68106-5915, USA. Tel: +1-402-559-8187, E-mail: cgurumurthy@unmc.edu; Thomas L. Saunders, Department of Internal Medicine, Division of Genetic Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Tel: +1-734-647-2910, E-mail: tsaunder@umich.edu; Masato Ohtsuka, Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. Tel: +81-463-93-1121, E-mail: masato@is.icc.u-tokai.ac.jp
  • Received: 2020-11-28
  • Revised: 2021-02-26
  • Accepted: 2021-03-10
  • Published: 2021-03-26
  • Issue Date: 2021-03-28
  • Genetically engineered mouse (GEM) models are commonly used in biomedical research. Generating GEMs involve complex set of experimental procedures requiring sophisticated equipment and highly skilled technical staff. Because of these reasons, most research institutes set up centralized core facilities where custom GEMs are created for research groups. Researchers, on the other hand, when they begin thinking about generating GEMs for their research, several questions arise in their minds. For example, what type of model(s) would be best useful for my research, how do I design them, what are the latest technologies and tools available for developing my model(s), and finally how to breed GEMs in my research. As there are several considerations and options in mouse designs, and as it is an expensive and time-consuming endeavor, careful planning upfront can ensure the highest chance of success. In this article, we provide brief answers to several frequently asked questions that arise when researchers begin thinking about generating mouse model(s) for their work.


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