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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 35 Issue 2
Mar.  2021
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Lyu Qing Rex, Yao Peng, Miano Joseph M.. Generating a CRISPR knockout mouse through a strong premature termination codon: a cautionary tale[J]. The Journal of Biomedical Research, 2021, 35(2): 174-178. DOI: 10.7555/JBR.34.20200106
Citation: Lyu Qing Rex, Yao Peng, Miano Joseph M.. Generating a CRISPR knockout mouse through a strong premature termination codon: a cautionary tale[J]. The Journal of Biomedical Research, 2021, 35(2): 174-178. DOI: 10.7555/JBR.34.20200106
shu

Generating a CRISPR knockout mouse through a strong premature termination codon: a cautionary tale

  • 1.

    Aab Cardiovascular Research Institute, University of Rochester School of Medicine & Dentistry, Rochester, NY 14642, USA

  • 2.

    Vascular Biology Center, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA

  • 3.

    Institute of Biomedicine and Health, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China

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  • Corresponding author:

    Tel: +86-18575532263, E-mail: lvqing@cigit.ac.cn

  • Received Date: July 10, 2020
  • Revised Date: November 08, 2020
  • Accepted Date: November 15, 2020
  • Available Online: December 24, 2020
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    • References (10)
  • [1]
    Miano JM, Zhu QM, Lowenstein CJ. A CRISPR path to engineering new genetic mouse models for cardiovascular research[J]. Arterioscler Thromb Vasc Biol,2016, 36(6): 1058–1075. doi: 10.1161/ATVBAHA.116.304790
    [2]
    McCaughan KK, Brown CM, Dalphin ME, et al. Translational termination efficiency in mammals is influenced by the base following the stop codon[J].Proc Natl Acad Sci U S A,1995, 92(12): 5431–5435. doi: 10.1073/pnas.92.12.5431
    [3]
    Yu M, Le SM, Ammon YC, et al. Force-dependent regulation of talin-KANK1 complex at focal adhesions[J]. Nano Lett,2019, 19(9): 5982–5990. doi: 10.1021/acs.nanolett.9b01732
    [4]
    Zhao JJ, Zhang W, Lin MY, et al. MYOSLID is a novel serum response factor-dependent long noncoding RNA that amplifies the vascular smooth muscle differentiation program[J]. Arterioscler Thromb Vasc Biol,2016, 36(10): 2088–2099. doi: 10.1161/ATVBAHA.116.307879
    [5]
    Jungreis I, Lin MF, Spokony R, et al. Evidence of abundant stop codon readthrough in Drosophila and other metazoa[J]. Genome Res,2011, 21(12): 2096–2113. doi: 10.1101/gr.119974.110
    [6]
    Cohen S, Kramarski L, Levi S, et al. Nonsense mutation-dependent reinitiation of translation in mammalian cells[J]. Nucleic Acids Res,2019, 47(12): 6330–6338. doi: 10.1093/nar/gkz319
    [7]
    Kurosaki T, Popp MW, Maquat LE. Quality and quantity control of gene expression by nonsense-mediated mRNA decay[J]. Nat Rev Mol Cell Biol,2019, 20(7): 406–420. doi: 10.1038/s41580-019-0126-2
    [8]
    Smits AH, Ziebell F, Joberty G, et al. Biological plasticity rescues target activity in CRISPR knock outs[J]. Nat Methods,2019, 16(11): 1087–1093. doi: 10.1038/s41592-019-0614-5
    [9]
    Smyth JW, Shaw RM. Autoregulation of connexin43 gap junction formation by internally translated isoforms[J]. Cell Rep,2013, 5(3): 611–618. doi: 10.1016/j.celrep.2013.10.009
    [10]
    Popp MW, Maquat LE. Leveraging rules of nonsense-mediated mRNA decay for genome engineering and personalized medicine[J]. Cell,2016, 165(6): 1319–1322. doi: 10.1016/j.cell.2016.05.053
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