4.6

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2.2

Impact Factor
  • ISSN 1674-8301
  • CN 32-1810/R
Rakesh Kumar Tekade, Antony D'Emanuele, Abdelbary Elhissi, Ashish Agrawal, Anurekha Jain, Basel Tawfiq Arafat, Narendra Kumar Jain. Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples[J]. The Journal of Biomedical Research, 2013, 27(5): 394-405. DOI: 10.7555/JBR.27.20120123
Citation: Rakesh Kumar Tekade, Antony D'Emanuele, Abdelbary Elhissi, Ashish Agrawal, Anurekha Jain, Basel Tawfiq Arafat, Narendra Kumar Jain. Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples[J]. The Journal of Biomedical Research, 2013, 27(5): 394-405. DOI: 10.7555/JBR.27.20120123

Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples

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This work was supported by Senior Research Fellowship and Commonwealth Split Site Fellowship awards from the Council for Scientific Industrial Research (CSIR), New Delhi (India) and the Association of Commonwealth Universities, UK, respectively.

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  • Received Date: January 13, 2013
  • A rapid, sensitive, selective and validated reverse phase high-performance liquid chromatography (RP-HPLC) method for the estimation of paclitaxel in micro-sample of rat plasma and in culture of cancer cells was per?formed in this study. The mobile phase consisted of an optimized mixture of methanol:water: trifluroacetic acid (80: 20: 0.1, v/v/v). Column elution at a flow rate of 1 mL/minute with UV detection at 225 nm at room tem?perature was used. The RP-HPLC method was successfully applied for the determination of paclitaxel in plasma samples and in culture of cancer cells with nano-quantity of estimation. The validation studies were performed in accordance with the International Conference on Harmonization (ICH) guidelines. The intra- and inter-day pre?cision showed that the coefficients of variation ranged from 1.07% to 4.27% at different levels of concentrations. To the best of our knowledge, this study also reported for the first time the optimization of different solvents for effective extraction of paclitaxel wherein tert.-butyl methyl ether (TBME): diethyl ether (DEE) in 50: 50 v/ v composition was found most efficient with extraction efficiency ranging between 77.99% and 91.74% and be?tween 76.14 and 93.66% in the plasma and cell culture, respectively. This proposed method was successfully ap?plied to study the pharmacokinetics of paclitaxel and the influence of verapamil and all-trans retinoic acid (atRA) on paclitaxel pharmacokinetics in rat models. This proposed method might emerge as a valuable aid in the labo?ratory monitoring of paclitaxel in a variety of in vitro as well as in vivo scenarios.
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