Hong Li, Chunyan Gu, Yongya Ren, Yang Dai, Xiaojuan Zhu, Jing Xu, Yuhua Li, Zhenning Qiu, Jin Zhu, Yinchang Zhu, Xiaohong Guan, Zhenqing Feng. The efficacy of NP11-4-derived immunotoxin scFv-artesunate in reducing hepatic fibrosis induced by Schistosoma japonicum in mice[J]. The Journal of Biomedical Research, 2011, 25(2): 148-154. DOI: 10.1016/S1674-8301(11)60019-5
Citation:
Hong Li, Chunyan Gu, Yongya Ren, Yang Dai, Xiaojuan Zhu, Jing Xu, Yuhua Li, Zhenning Qiu, Jin Zhu, Yinchang Zhu, Xiaohong Guan, Zhenqing Feng. The efficacy of NP11-4-derived immunotoxin scFv-artesunate in reducing hepatic fibrosis induced by Schistosoma japonicum in mice[J]. The Journal of Biomedical Research, 2011, 25(2): 148-154. DOI: 10.1016/S1674-8301(11)60019-5
Hong Li, Chunyan Gu, Yongya Ren, Yang Dai, Xiaojuan Zhu, Jing Xu, Yuhua Li, Zhenning Qiu, Jin Zhu, Yinchang Zhu, Xiaohong Guan, Zhenqing Feng. The efficacy of NP11-4-derived immunotoxin scFv-artesunate in reducing hepatic fibrosis induced by Schistosoma japonicum in mice[J]. The Journal of Biomedical Research, 2011, 25(2): 148-154. DOI: 10.1016/S1674-8301(11)60019-5
Citation:
Hong Li, Chunyan Gu, Yongya Ren, Yang Dai, Xiaojuan Zhu, Jing Xu, Yuhua Li, Zhenning Qiu, Jin Zhu, Yinchang Zhu, Xiaohong Guan, Zhenqing Feng. The efficacy of NP11-4-derived immunotoxin scFv-artesunate in reducing hepatic fibrosis induced by Schistosoma japonicum in mice[J]. The Journal of Biomedical Research, 2011, 25(2): 148-154. DOI: 10.1016/S1674-8301(11)60019-5
Key Labortary of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, Jiangsu 210029, China/Pathology Department, Nanjing Medical University , Nanjing, Jiangsu 210029, China
2.
Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu 214000, China
3.
Key Labortary of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, Jiangsu 210029, China
4.
Pathology Department, Nanjing Medical University , Nanjing, Jiangsu 210029, China
5.
Key Labortary of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, Jiangsu 210029, China/Huadong Medical Institute of Biotechniques, Nanjing, Jiangsu 210002, China
Funds:
This work was supported by a grant from the National High Tech-nology Research and Development Program of China ("863"Program No.2006AA02Z415).
Schistosomiasis is one of the most prevalent parasitic diseases in China, and hepatic fibrosis caused by schis-tosome infection is the principal cause of death. The aim of this study was to evaluate the efficacy of NP11-4-derived immunotoxin scFv-artesunate on Schistosoma japonicum-induced hepatic fibrosis. A single-chain variable fragment (scFv) was generated from the murine anti-Schistosoma japonicum (S. japanicum) monoclonal antibody NP11-4. The scFv was expressed as a soluble protein and purified by Ni-affinity chromatography. After conjuga-tion with artesunate, the binding ability with soluble egg antigens (SEA) was determined by an enzyme-linked immunosorbent assay (ELISA). The biological activity of purified scFv, scFv-artesunate (immunotoxin), and artesunate was detected in vivo. Image-Pro Plus software was used to analyze the size of egg granuloma and the extent of liver fibrosis. The recombinant scFv expession vector was constructed and expressed successfully. Af-ter purification by a His-trap Ni-affinity column, the scFv yield was approximately 0.8 mg/L of culture medium. ELISA results showed that chemical conjugation did not affect the binding activity of the immunotoxin. Our ani-mal experiments indicated that the immunotoxin could significantly reduce the size of egg granuloma in the liver and inhibit hepatic fibrosis. The immunotoxin could be used as a promising candidate in the targeted therapy of S. japonicum-induced hepatic fibrosis.