Designing and generating a mouse model: frequently asked questions

Gurumurthy Channabasavaiah B.; Saunders Thomas L.; Ohtsuka Masato

doi:10.7555/JBR.35.20200197

Volume 35 Issue 2

Mar. 2021

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Abstract

References

The Journal of Biomedical Research > 2021 > 35(2): 76-90. > DOI: 10.7555/JBR.35.20200197

Gurumurthy Channabasavaiah B., Saunders Thomas L., Ohtsuka Masato. Designing and generating a mouse model: frequently asked questions[J]. The Journal of Biomedical Research, 2021, 35(2): 76-90. DOI: 10.7555/JBR.35.20200197

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Designing and generating a mouse model: frequently asked questions

Gurumurthy Channabasavaiah B.^{1, 2, ,},
Saunders Thomas L.^{3, 4, ,},
Ohtsuka Masato^{5, 6, ,}

1.
Department of Pharmacology and Experimental Neuroscience
2.
Mouse Genome Engineering Core Facility, Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68106-5915, USA
3.
Department of Internal Medicine, Division of Genetic Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA
4.
Transgenic Animal Model Core, University of Michigan, Ann Arbor, MI 48109, USA
5.
Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan
6.
The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa 259-1193, Japan

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Corresponding author:
Channabasavaiah B. Gurumurthy, Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68106-5915, USA. Tel: +1-402-559-8187, E-mail: cgurumurthy@unmc.edu

Thomas L. Saunders, Department of Internal Medicine, Division of Genetic Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Tel: +1-734-647-2910, E-mail: tsaunder@umich.edu

Masato Ohtsuka, Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. Tel: +81-463-93-1121, E-mail: masato@is.icc.u-tokai.ac.jp
Received Date: November 27, 2020
Revised Date: February 25, 2021
Accepted Date: March 09, 2021
Available Online: March 25, 2021

Graphical Abstract

Abstract

Abstract

Genetically engineered mouse (GEM) models are commonly used in biomedical research. Generating GEMs involve complex set of experimental procedures requiring sophisticated equipment and highly skilled technical staff. Because of these reasons, most research institutes set up centralized core facilities where custom GEMs are created for research groups. Researchers, on the other hand, when they begin thinking about generating GEMs for their research, several questions arise in their minds. For example, what type of model(s) would be best useful for my research, how do I design them, what are the latest technologies and tools available for developing my model(s), and finally how to breed GEMs in my research. As there are several considerations and options in mouse designs, and as it is an expensive and time-consuming endeavor, careful planning upfront can ensure the highest chance of success. In this article, we provide brief answers to several frequently asked questions that arise when researchers begin thinking about generating mouse model(s) for their work.
- CRISPR,
- transgenic mouse,
- genetic engineering,
- knockout mouse,
- conditional knockout mouse,
- knock-in mouse

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References (87)

References

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