4.6

CiteScore

2.2

Impact Factor
  • ISSN 1674-8301
  • CN 32-1810/R
Hamid A Bakshi, Faruck Lukmanul Hakkim, Smitha Sam, Farideh Javid, Luay Rashan. Dietary crocin reverses melanoma metastasis[J]. The Journal of Biomedical Research, 2018, 32(1): 39-50. DOI: 10.7555/JBR.31.20160120
Citation: Hamid A Bakshi, Faruck Lukmanul Hakkim, Smitha Sam, Farideh Javid, Luay Rashan. Dietary crocin reverses melanoma metastasis[J]. The Journal of Biomedical Research, 2018, 32(1): 39-50. DOI: 10.7555/JBR.31.20160120

Dietary crocin reverses melanoma metastasis

More Information
  • Received Date: September 25, 2016
  • Revised Date: February 16, 2017
  • Crocus sativus and its bioactive constituent crocin are well known for anti-tumor potential in different models. However, the efficacy of crocin on in-vivo melanoma metastasis is not yet reported. In this study, melanoma metastatic model was developed by tail vein injection of B16F-10 cells in to C57BL/6 mice. Metastatic mice treated with two different doses of crocin (250 and 500 μg/kg of bodyweight) for 10 days and parameters such as lung metastasis inhibition, mean survival time, lung hydroxyproline, uronic acid and hexosamine levels were analyzed after 21 days of treatment. Then blood was collected and serum gamma glutamyl transpeptidase (g-GGT), sialic acid, tumor necrosis factor alpha (TNF-α), interleukin 10 (IL-10), IL-6, IL-2, and TIMP-1 levels were measured. Further, a lung histological examination was done in crocin treated metastatic mice. Subsequently hallmark metastatic parameters such as matrix metalloproteinases (MMPs), extracellular regulated kinase 2 (ERK2), vascular endothelial growth factor (VEGF), and K-ras gene expression were investigated in the lungs of crocin treated metastatic mice. Further, in-vitro adhesion, invasion and migration of B16F-10 cells were examined after 24 hours of crocin (5 and 10 μg/mL) treatment. Administration of crocin to tumor bearing C57BL/6 mice reduced the lung metastasis by 85%. Elevated levels of hydroxyproline, uronic acid, hexosamine, serum sialic acid and g-GGT in metastatic control were found to be significantly reduced in crocin treated mice. Crocin also inhibited expression of MMP-2, MMP-9, ERK-2, K-ras, and VEGF. Crocin reduced the ability of B16F-10 cells invasion (P < 0.05), migration (P < 0.05) and adhesion by upregulating E-cadherin expression. In conclusion, crocin elicited marked anti-metastatic potential by regulating the metastasis induced biomarkers.
  • Related Articles

    [1]Chanjuan Gong, Xiaokai Zhou, Yin Fang, Yanjuan Zhang, Linjia Zhu, Zhengnian Ding. Effects of sevoflurane on left ventricular function by speckle-tracking echocardiography in coronary bypass patients: A randomized trial[J]. The Journal of Biomedical Research, 2024, 38(1): 76-86. DOI: 10.7555/JBR.37.20230173
    [2]Mingfeng He, Chanjuan Gong, Yinan Chen, Rongting Chen, Yanning Qian. Effect of remimazolam vs. propofol on hemodynamics during general anesthesia induction in elderly patients: Single-center, randomized controlled trial[J]. The Journal of Biomedical Research, 2024, 38(1): 66-75. DOI: 10.7555/JBR.37.20230110
    [3]Kaibo Lin, Shikun Zhang, Jieli Chen, Ding Yang, Mengyi Zhu, Eugene Yujun Xu. Generation and functional characterization of a conditional Pumilio2 null allele[J]. The Journal of Biomedical Research, 2018, 32(6): 434-441. DOI: 10.7555/JBR.32.20170117
    [4]Minglong Chen. Ventricular tachycardia ablation and substrate modification in ICD patients with electrical storm[J]. The Journal of Biomedical Research, 2015, 29(1): 52-55. DOI: 10.7555/JBR.29.20140165
    [5]Akihiko Nogami. Trigger elimination of polymorphic ventricular tachycardia and ventricular fibrillation by catheter ablation: trigger and substrate modification[J]. The Journal of Biomedical Research, 2015, 29(1): 44-51. DOI: 10.7555/JBR.29.20140156
    [6]Zhiyong Qian, Jianghong Guo, Zhiyong Zhang, Yao Wang, Xiaofeng Hou, Jiangang Zou. Optimal programming management of ventricular tachycardia storm in ICD patients[J]. The Journal of Biomedical Research, 2015, 29(1): 35-43. DOI: 10.7555/JBR.29.20140146
    [7]Wei Qin, Liming Wang, Xin Chen, Peisheng Liu, Rui Wang. Left ventricular myxoma: a case report[J]. The Journal of Biomedical Research, 2014, 28(6): 506-508. DOI: 10.7555/JBR.28.20120124
    [8]Weihua Zhou, Ji Chen. I -123 metaiodobenzylguanidine imaging for predicting ventricular arrhythmia in heart failure patients[J]. The Journal of Biomedical Research, 2013, 27(6): 460-466. DOI: 10.7555/JBR.27.20130137
    [9]Chunyan Cheng, Yuan Lin, Fan Yang, Wenjing Wang, Chong Wu, Jingli Qin, Xiuqin Shao, Lei Zhou. Mutational screening of affected cardiac tissues and peripheral blood cells identified novel somatic mutations in GATA4 in patients with ventricular septal defect[J]. The Journal of Biomedical Research, 2011, 25(6): 425-430. DOI: 10.1016/S1674-8301(11)60056-0
    [10]Zhao Zhao, Zuyi Yuan, Yuqiang Ji, Yue Wu, Yinzhi Qi. Left ventricular hypertrophy amplifies the QT, and Tp-e intervals and the Tp-e/ QT ratio of left chest ECG[J]. The Journal of Biomedical Research, 2010, 24(1): 69-72.
  • Cited by

    Periodical cited type(1)

    1. Shen Q, Li J, Cui C, et al. A wearable real-time telemonitoring electrocardiogram device compared with traditional Holter monitoring. J Biomed Res, 2020, 35(3): 238-246. DOI:10.7555/JBR.34.20200074

    Other cited types(0)

Catalog

    Article Metrics

    Article views PDF downloads Cited by(1)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return