Tanja Stachon, Jiong Wang, Xufei Song, Achim Langenbucher, Berthold Seitz, No´ra Szentma´ry. Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on
viability, apoptosis and activation of human keratocytes in vitro[J]. The Journal of Biomedical Research, 2015, 29(4): 321-325. DOI: 10.7555/JBR.29.20130173
Citation:
Tanja Stachon, Jiong Wang, Xufei Song, Achim Langenbucher, Berthold Seitz, No´ra Szentma´ry. Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on
viability, apoptosis and activation of human keratocytes in vitro[J]. The Journal of Biomedical Research, 2015, 29(4): 321-325. DOI: 10.7555/JBR.29.20130173
Tanja Stachon, Jiong Wang, Xufei Song, Achim Langenbucher, Berthold Seitz, No´ra Szentma´ry. Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on
viability, apoptosis and activation of human keratocytes in vitro[J]. The Journal of Biomedical Research, 2015, 29(4): 321-325. DOI: 10.7555/JBR.29.20130173
Citation:
Tanja Stachon, Jiong Wang, Xufei Song, Achim Langenbucher, Berthold Seitz, No´ra Szentma´ry. Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on
viability, apoptosis and activation of human keratocytes in vitro[J]. The Journal of Biomedical Research, 2015, 29(4): 321-325. DOI: 10.7555/JBR.29.20130173
Riboflavin-UVA photodynamic inactivation is a potential treatment alternative in therapy resistant infectious keratitis.
The purpose of our study was to determine the impact of riboflavin-UVA photodynamic inactivation on viability, apop-
tosis and activation of human keratocytes in vitro. Primary human keratocytes were isolated from human corneal buttons
and cultured in DMEM/Ham9s F12 medium supplemented with 10% fetal calf serum. Keratocytes underwent UVA light
illumination (375 nm) for 4.10 minutes (2 J/cm
2) during exposure to different concentrations of riboflavin. Twenty-four
hours after treatment, cell viability was evaluated photometrically, whereas apoptosis, CD34 and alpha-smooth muscle
actin (a-SMA) expression were assessed using flow cytometry. We did not detect significant changes in cell viability,
apoptosis, CD34 and a-SMA expression in groups only treated with riboflavin or UVA light. In the group treated with
riboflavin-UVA-photodynamic inactivation, viability of keratocytes decreased significantly at 0.1% riboflavin (P,0.01)
while the percentage of CD34 (P,0.01 for both 0.05% and 0.1% riboflavin) and alpha-SMA positive keratocytes
(P,0.01 and P,0.05 for 0.05% and 0.1% riboflavin, respectively) increased significantly compared to the controls.
There was no significant change in the percentage of apoptotic keratocytes compared to controls at any of the used ribo-
flavin concentrations (P50.09 and P50.13). We concluded that riboflavin-UVA-photodynamic-inactivation decreases
viability of myofibroblastic transformation and multipotent haematopoietic stem cell transformation; however, it does
not have an impact on apoptosis of human keratocytes in vitro.
Liukkonen M, Heloterä H, Siintamo L, et al. Oxidative Stress and Inflammation-Related mRNAs Are Elevated in Serum of a Finnish Wet AMD Cohort. Invest Ophthalmol Vis Sci, 2024, 65(13): 30.
DOI:10.1167/iovs.65.13.30
1.
Liukkonen M, Heloterä H, Siintamo L, et al. Oxidative Stress and Inflammation-Related mRNAs Are Elevated in Serum of a Finnish Wet AMD Cohort. Invest Ophthalmol Vis Sci, 2024, 65(13): 30.
DOI:10.1167/iovs.65.13.30