4.6

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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 25 Issue 5
Aug.  2011
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Ali Ahadi, Alireza Partoazar, Mohammad-Hassan Abedi-Khorasgani, Seyed Vahid Shetab-Boushehri. Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine[J]. The Journal of Biomedical Research, 2011, 25(5): 362-367. DOI: 10.1016/S1674-8301(11)60048-1
Citation: Ali Ahadi, Alireza Partoazar, Mohammad-Hassan Abedi-Khorasgani, Seyed Vahid Shetab-Boushehri. Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine[J]. The Journal of Biomedical Research, 2011, 25(5): 362-367. DOI: 10.1016/S1674-8301(11)60048-1
shu

Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

  • 1.

    Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. 1449614535 Iran

  • 2.

    Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. 1449614535 Iran

  • 3.

    Legal Medicine Organization of Iran, Tehran, I. R.1449614535

  • 4.

    Iran.

  • 5.

    Department of Medical Nanotechnology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. 1449614535 Iran

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  • Received Date: May 21, 2011
    Graphical Abstract
    • Abstract
  • Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the tra-ditional LLE method. High performance thin layer chromatography (HPTLC) has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over that of a routine LLE-TLC method, in extraction and detection of urinary morphine. Fifty-eight urine samples, primarily identified as mor-phine-positive samples by a strip test, were re-screened by LLE-TLC and SPE-HPTLC. The results of LLE-TLC and SPE-HPTLC were then compared with each other. The results showed that the SPE-HPTLC detected 74% of total samples as morphine-positive samples whereas the LLE-TLC detected 48% of the same samples. We further discussed the effect of codeine abuse on TLC analysis of urinary morphine. Regarding the importance of morphine detection in urine, the present combined SPE-HPTLC method is suggested as a replacement method for detection of urinary morphine by many reference laboratories.
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