Lingyun Li, Jing Chi, Feng Zhou, Dandan Guo, Fang Wang, Genyan Liu, Chun Zhang, Kun Yao. Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway[J]. The Journal of Biomedical Research, 2010, 24(6): 444-451. DOI: 10.1016/S1674-8301(10)60059-0
Citation:
Lingyun Li, Jing Chi, Feng Zhou, Dandan Guo, Fang Wang, Genyan Liu, Chun Zhang, Kun Yao. Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway[J]. The Journal of Biomedical Research, 2010, 24(6): 444-451. DOI: 10.1016/S1674-8301(10)60059-0
Lingyun Li, Jing Chi, Feng Zhou, Dandan Guo, Fang Wang, Genyan Liu, Chun Zhang, Kun Yao. Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway[J]. The Journal of Biomedical Research, 2010, 24(6): 444-451. DOI: 10.1016/S1674-8301(10)60059-0
Citation:
Lingyun Li, Jing Chi, Feng Zhou, Dandan Guo, Fang Wang, Genyan Liu, Chun Zhang, Kun Yao. Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway[J]. The Journal of Biomedical Research, 2010, 24(6): 444-451. DOI: 10.1016/S1674-8301(10)60059-0
Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, Jiangsu 210029, China/Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China
2.
Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, Jiangsu 210029, China
3.
Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
Funds:
This work was supported by the National Natural Science Foundation of China (No. 30771961 and No. 30901344), Science Development Foundation of Nanjing Medical University (No. 08NMUZ003) and Jiangsu Province Laboratory of Pathogen Biology (No. 08bykf01)
Apoptosis plays an important role in the pathogenesis of viral infections. In this study, we investigated the cell death processes during productive HHV-6A infection and the underlying mechanisms. Annexin V-PI staining and electron microscopy indicated that HHV-6A is a strong inducer of apoptosis. HHV-6A infection decreased mi-tochondrial transmembrane potential and led to morphological changes of mitochondria. The cell death was as-sociated with activation of caspase-3 and cleavage of DNA repair enzyme poly (ADP-ribose) polymerase, which is known to be an important substrate for activated caspase-3. Caspase-9 was activated significantly in HHV-6A-infected cells, whereas caspase-8 was not activated obviously. Moreover, HHV-6A infection upregulated Bax and downregulated Bcl-2. This is the first demonstration of mitochondrion-mediated, caspase-dependent apoptosis in HHV-6A-infected cells.
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