4.6

CiteScore

2.2

Impact Factor
  • ISSN 1674-8301
  • CN 32-1810/R
Volume 24 Issue 6
Oct.  2010
Turn off MathJax
Article Contents
Abstract
Related Articles
Ping Li, Xiaoming Lu, Yingyi Wang, Lihua Sun, Chunfa Qian, Wei Yan, Ning Liu, Yongping You, Zhen Fu. MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells[J]. The Journal of Biomedical Research, 2010, 24(6): 436-443. DOI: 10.1016/S1674-8301(10)60058-9
Citation: Ping Li, Xiaoming Lu, Yingyi Wang, Lihua Sun, Chunfa Qian, Wei Yan, Ning Liu, Yongping You, Zhen Fu. MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells[J]. The Journal of Biomedical Research, 2010, 24(6): 436-443. DOI: 10.1016/S1674-8301(10)60058-9
shu

MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells

  • 1.

    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China

  • 2.

    Department of Neurosurgery, the Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China

Funds: 

This work is supported by the China Natural Science Foundation (No. 30872657), Jiangsu Province’s Medical Major Talent Foundation (No. RC2007061), Jiangsu Province’s Natural Science Foundation (No. 2008475) and Jiangsu Province’s "333" Key Talent Foundati

More Information
  • Received Date: July 14, 2010
    Graphical Abstract
    • Abstract
  • MicroRNAs regulate self renewal and differentiation of cancer stem cells. There, we sought to identify the ex-pression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study. MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting. After miR-181b was overexpressed in U87 glioma stem cells by miR-181b lentiviral expression vector and/or treatment of temozolomide, secondary neurosphere assay, soft agar colony assay and MTT assay were performed. Compared with U87 cells, the expression of miR-181b was significantly decreased in U87 glioma stem cells. Overexpression of miR-181b decreased neurosphere formation by U87 glioma stem cells in vitro and suppressed colony formation in soft agar, and the cell growth inhibition rates increased in a time-dependent man-ner in U87 glioma stem cells infected with miR-181b lentivirus. Furthermore, miR-181b had a synergistic effect on temozolomide-induced inhibition of secondary neurosphere and soft agar colony, and on cell growth inhibition rates. MiR-181b functions as a tumor suppressor that suppresses proliferation and reduces chemoresistance to te-mozolomide in glioma stem cells.
    • FullText(HTML)
    • References (0)
    • Related Articles

  • Related Articles

    [1]Juan Zhou, Yiran Xu, Luyao Wang, Yu Cong, Ke Huang, Xinxing Pan, Guangquan Liu, Wenqu Li, Chenchen Dai, Pengfei Xu, Xuemei Jia. LncRNA IDH1-AS1 sponges miR-518c-5p to suppress proliferation of epithelial ovarian cancer cell by targeting RMB47[J]. The Journal of Biomedical Research, 2024, 38(1): 51-65. DOI: 10.7555/JBR.37.20230097
    [2]Wang Jing, He Xuezhi, Lu Xiyi, Amin Karim Muhammad, Miao Dengshun, Zhang Erbao. A novel long non-coding RNA NFIA-AS1 is down-regulated in gastric cancer and inhibits proliferation of gastric cancer cells[J]. The Journal of Biomedical Research, 2019, 33(6): 371-381. DOI: 10.7555/JBR.33.20190015
    [3]Zhu Chenchen, Jiang Haonan, Deng Wenjie, Zhao Shuo, Li Kaiquan, Wang Yuting, Wei Qinjun, Du Jun. Activation of p38/HSP27 pathway counters melatonin-induced inhibitory effect on proliferation of human gastric cancer cells[J]. The Journal of Biomedical Research, 2019, 33(5): 317-324. DOI: 10.7555/JBR.33.20180066
    [4]Xu Yuyu, Wang Pengqi, Xu Chaoqi, Shan Xiaoyun, Feng Qing. Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression[J]. The Journal of Biomedical Research, 2019, 33(3): 181-191. DOI: 10.7555/JBR.31.20170016
    [5]Lintao Wang, Yanyan Peng, Kaikai Shi, Haixiao Wang, Jianlei Lu, Yanli Li, Changyan Ma. Osthole inhibits proliferation of human breast cancer cells by inducing cell cycle arrest and apoptosis[J]. The Journal of Biomedical Research, 2015, 29(2): 132-138. DOI: 10.7555/JBR.27.20120115
    [6]Ning Shi, Shi-You Chen. Mechanisms simultaneously regulate smooth muscle proliferation and differentiation[J]. The Journal of Biomedical Research, 2014, 28(1): 40-46. DOI: 10.7555/JBR.28.20130130
    [7]Mengying Liu, Yao Hu, Lijuan Zhu, Chen Chen, Yu Zhang, Weixiang Sun, Qigang Zhou. Overexpression of the mTERT gene by adenoviral vectors promotes the proliferation of neuronal stem cells in vitro and stimulates neurogenesis in the hippocampus of mice[J]. The Journal of Biomedical Research, 2012, 26(5): 381-388. DOI: 10.7555/JBR.26.20110078
    [8]Tian Tian, Yajie Zhang, Shouyu Wang, Jianwei Zhou, Shan Xua. Sox2 enhances the tumorigenicity and chemoresistance of cancer stem-like cells derived from gastric cancer[J]. The Journal of Biomedical Research, 2012, 26(5): 336-345. DOI: 10.7555/JBR.26.20120045
    [9]Wen Qiu, Yan Li, Jianbo Zhou, Chenhui Zhao, Jing Zhang, Kai Shan, Dan Zhao, Yingwei Wang. TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats[J]. The Journal of Biomedical Research, 2011, 25(6): 402-410. DOI: 10.1016/S1674-8301(11)60053-5
    [10]Zhengxian Tao, Bo Chen, Yingming Zhao, Hongwu Chen, Liansheng Wang, Yonghong Yong, Kejiang Cao, Qifeng Yu, Danian Ke, Hua Wang, Zuze Wu, Zhijian Yang. HGF percutaneous endocardial injection induces cardiomyocyte proliferation and rescues cardiac function in pigs[J]. The Journal of Biomedical Research, 2010, 24(3): 198-206.

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (4239) PDF downloads (1299) Cited by()
    Related

    ©2023 by the Editorial Department of the Journals of Nanjing Medical University. All rights reserved.   苏ICP备05071376号-5

    Supported by: Beijing Renhe Information Technology Co., Ltd. E-mail: info@rhhz.net  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return