• ISSN 1674-8301
  • CN 32-1810/R
Volume 30 Issue 4
Jul.  2016
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Article Contents
Yeong-Min Yoo, Su Kil Jang, Gwang-Hoon Kim, Jung-Youl Park, Seong-Soo Joo. Pharmacological advantages of melatonin in immunosenescence by improving activity of T lymphocytes[J]. The Journal of Biomedical Research, 2016, 30(4): 314-321. doi: 10.7555/JBR.30.2016K0010
Citation: Yeong-Min Yoo, Su Kil Jang, Gwang-Hoon Kim, Jung-Youl Park, Seong-Soo Joo. Pharmacological advantages of melatonin in immunosenescence by improving activity of T lymphocytes[J]. The Journal of Biomedical Research, 2016, 30(4): 314-321. doi: 10.7555/JBR.30.2016K0010

Pharmacological advantages of melatonin in immunosenescence by improving activity of T lymphocytes

doi: 10.7555/JBR.30.2016K0010
Funds:

High Value-added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA

grant number 113034-3)

  • Received: 2015-08-18
  • Revised: 2015-11-05
  • Issue Date: 2016-07-28
  • Melatonin plays a critical role in regulating photoperiodic signals and has recently been shown to decrease immunosenescence with age. In this study, we examined whether melatonin activates T lymphocytes as major adaptive immune cells in in vitro and in vivo models. Splenocytes, CD4+, and naive CD4 T lymphocytes were isolated from the spleen of BALB/c mice and the cell population patterns and mRNA profiles associated with T cell activation (CD28 and p21) and the melatonin receptor (MT1A and MT1B) were assessed. The T cell activationrelated proteins Ki67 and Bcl2 were also evaluated to confirm the relationship between gene and protein levels. Our data clearly revealed that CD28, p21, MT1A, and MT1B mRNA were highly expressed in the presence of melatonin.Co-culture of CD4+ T lymphocyte and peritoneal macrophage 7 days after melatonin administration to young and aged mice significantly increased APRIL mRNA, suggesting induction or maintenance of T lymphocyte response We also found that the intracellular amount of Ki67 and Bcl2 proteins were significantly upregulated in aged CD4+ Tlymphoc^es, suggesting enhancing T cell proliferation and ling-term maintenance of memory T cells. Taken together, we conclude that melatonin supplementation may enhance immunity in aged individuals by upregulating immunosenescence indices in association with T lymphocytes and may be an attractive pharmacological candidate for aged and immunocompromised individuals.

     

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