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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 29 Issue 5
Aug.  2015
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Article Contents
Abstract
Xianjin Ke, Qian Li, Li Xu, Ying Zhang, Dongmei Li, Jianhua Ma, Xiaoming Mao. Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury[J]. The Journal of Biomedical Research, 2015, 29(5): 380-389. DOI: 10.7555/JBR.29.20140076
Citation: Xianjin Ke, Qian Li, Li Xu, Ying Zhang, Dongmei Li, Jianhua Ma, Xiaoming Mao. Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury[J]. The Journal of Biomedical Research, 2015, 29(5): 380-389. DOI: 10.7555/JBR.29.20140076
shu

Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury

  • 1.

    Department of Neurology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China

  • 2.

    Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China

Funds: 

Jiangsu Planned Projects for Postdoctoral Research Funds, Nanjing Medical Technology Development Project (No. ZKX08014), Nanjing Medical Science and Technique Development Foundation, National Natural Science Foundation of China (No. 81200594)

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  • Received Date: May 15, 2014
  • Revised Date: September 30, 2014
    Graphical Abstract
    • Abstract
  • Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been developed as a new method of treating diseases of the peripheral nervous system. While netrin-1 is a critical molecule for axonal path finding and nerve growth, it may also affect vascular network formation. Here, we investigated the effect of transplanting BMSCs that produce netrin-1 in a rat model of sciatic nerve crush injury. We introduced a sciatic nerve crush injury, and then injected 16106 BMSCs infected by a recombinant adenovirus expressing netrin-1 Ad5-Netrin-1-EGFP or culture medium into the injured part in the next day. At day 7, 14 and 28 after injection, we measured motor nerve conduction and detected mRNA expressions of netrin-1 receptors UNC5B and Deleted in Colorectal Cancer (DCC), and neurotrophic factors brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) by real-time PCR. We also detected protein expressions of BDNF and NGF by Western blotting assays and examined BMSCs that incorporated into myelin and vascellum. The results showed that BMSCs infected by Ad5-Netrin- 1-EGFP significantly improved the function of the sciatic nerve, and led to increased expression of BDNF and NGF (P,0.05). Moreover, 28 days after injury, more Schwann cells were found in BMSCs infected by Ad5- Netrin-1-EGFP compared to control BMSCs. In conclusion, transplantation of BMSCs that produce netrin-1 improved the function of the sciatic nerve after injury. This method may be a new treatment of nerve injury.
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