Regulatory role of sorting nexin 5 in protein stability and vesicular targeting of vesicular acetylcholine transporter to synaptic vesicle-like vesicles in PC12 cells

Sun Meihen; Han Xu; Chang Fei; Xu Hongfei; Colgan Lesley; Liu Yongjian

doi:10.7555/JBR.34.20200095

Volume 35 Issue 5

Sep. 2021

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The Journal of Biomedical Research > 2021 > 35(5): 339-350. > DOI: 10.7555/JBR.34.20200095

Sun Meihen, Han Xu, Chang Fei, Xu Hongfei, Colgan Lesley, Liu Yongjian. Regulatory role of sorting nexin 5 in protein stability and vesicular targeting of vesicular acetylcholine transporter to synaptic vesicle-like vesicles in PC12 cells[J]. The Journal of Biomedical Research, 2021, 35(5): 339-350. DOI: 10.7555/JBR.34.20200095

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Regulatory role of sorting nexin 5 in protein stability and vesicular targeting of vesicular acetylcholine transporter to synaptic vesicle-like vesicles in PC12 cells

Sun Meihen^{1, △},
Han Xu^{1, △},
Chang Fei^{2, △},
Xu Hongfei³,
Colgan Lesley^4, ,,
Liu Yongjian^1, ,

1.
Jiangsu Key Laboratory of Xenotransplantation, and Department of Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 211166, China
2.
Neuroscience Program, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
3.
Department of Neurology, University of California San Francisco School of Medicine, San Francisco, CA 94143, USA
4.
Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458, USA

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Corresponding author:
Yongjian Liu, Jiangsu Key Laboratory of Xenotransplantation, and Department of Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 211166, China. Tel: +86-25-86869442, E-mail: young.liu78@gmail.com

Lesley Colgan, Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458, USA. Tel: +1-561-972-9210, E-mail: lesley.col gan@nufi.org
Received Date: June 16, 2020
Revised Date: June 18, 2020
Accepted Date: June 18, 2020
Available Online: August 20, 2020

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Abstract

Abstract

Accurate targeting of vesicular acetylcholine transporter (VAChT) to synaptic vesicles (SVs) is indispensable for efficient cholinergic transmission. Previous studies have suggested that the dileucine motif within the C-terminus of the transporter is sufficient for its targeting to SVs. However, the cytosolic machinery underlying specific regulation of VAChT trafficking and targeting to SVs is still unclear. Here we used the C-terminus of VAChT as a bait in a yeast two-hybrid screen to identify sorting nexin 5 (SNX5) as its novel interacting protein. SNX5 was detected in the SVs enriched LP2 subcellular fraction of rat brain homogenate and showed strong colocalization with VAChT in both brain sections and PC12 cells. Binding assays suggested that the C-terminal domain of VAChT can interact with both BAR and PX domain of SNX5. Depletion of SNX5 enhanced the degradation of VAChT and the process was mediated through the lysosomal pathway. More importantly, we found that, in PC12 cells, the depletion of SNX5 expression significantly decreased the synaptic vesicle-like vesicles (SVLVs) localization of VAChT. Therefore, the results suggest that SNX5 is a novel regulator for both stability and SV targeting of VAChT.
- retrograde trafficking,
- vesicular targeting,
- synaptic vesicle-like vesicles,
- synaptic vesicles,
- PC12

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△ These authors contributed equally to this work

References (38)

References

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Regulatory role of sorting nexin 5 in protein stability and vesicular targeting of vesicular acetylcholine transporter to synaptic vesicle-like vesicles in PC12 cells