Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression

Xu Yuyu; Wang Pengqi; Xu Chaoqi; Shan Xiaoyun; Feng Qing

doi:10.7555/JBR.31.20170016

Volume 33 Issue 3

Apr. 2019

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The Journal of Biomedical Research > 2019 > 33(3): 181-191. > DOI: 10.7555/JBR.31.20170016

Xu Yuyu, Wang Pengqi, Xu Chaoqi, Shan Xiaoyun, Feng Qing. Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression[J]. The Journal of Biomedical Research, 2019, 33(3): 181-191. DOI: 10.7555/JBR.31.20170016

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Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression

Xu Yuyu^1,Δ,
Wang Pengqi^1,2,Δ,
Xu Chaoqi¹,
Shan Xiaoyun^1,3,
Feng Qing^1, ,

1.
Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China
2.
Station of Sanitary Surveillance of Lianyungang, Lianyungang, Jiangsu 222002, China
3.
University of South China, Hengyang, Hunan 421000, China

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Corresponding author:
Qing Feng, Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, 101 Longmian Avenue, Nanjing, Jiangsu 211166, China.Tel: +86- 25-86868455, Email:qingfeng@njmu.edu.cn
Received Date: February 15, 2017
Revised Date: March 28, 2017
Accepted Date: April 19, 2017
Available Online: May 26, 2017

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Abstract

Abstract

Acrylamide, a potential carcinogen, exists in carbohydrate-rich foods cooked at a high temperature. It has been reported that acrylamide can cause DNA damage and cytotoxicity. The present study aimed to investigate the potential mechanism of human hepatocarcinoma HepG2 cell proliferation induced by acrylamide and to explore the antagonistic effects of a natural polyphenol curcumin against acrylamide via miR-21. The results indicated that acrylamide (≤100 μmol/L) significantly increased HepG2 cell proliferation and miR-21 expression. In addition, acrylamide reduced the PTEN expression in protein level, while induced the expressions of p-AKT, EGFR and cyclin D1. The PI3K/AKT inhibitor decreased p-AKT protein expression and inhibited the proliferation of HepG2 cells. In addition, curcumin effectively reduced acrylamide-induced HepG2 cell proliferation and induced apoptosis through the expression of miR-21. In conclusion, the results showed that acrylamide increased HepG2 cell proliferation via upregulating miR-21 expression, which may be a new target for the treatment and prevention of cancer.
- acrylamide,
- proliferation,
- miR-21

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References

[1]	Parzefall W. Minireview on the toxicity of dietary acrylamide[J]. Food Chem Toxicol, 2008, 46(4): 1360-1364. doi: 10.1016/j.fct.2007.08.027
[2]	Tareke E, Rydberg P, Karlsson P, et al. Analysis of acrylamide, a carcinogen formed in heated foodstuffs[J]. J Agric Food Chem, 2002, 50(17): 4998-5006. doi: 10.1021/jf020302f
[3]	Pedreschi F, Mariotti MS, Granby K. Current issues in dietary acrylamide: formation, mitigation and risk assessment[J]. J Sci Food Agric, 2014, 94(1): 9-20. doi: 10.1002/jsfa.2014.94.issue-1
[4]	Fuhr U, Boettcher MI, Kinzig-Schippers M, et al. Toxicokinetics of acrylamide in humans after ingestion of a defined dose in a test meal to improve risk assessment for acrylamide carcinogenicity[J]. Cancer Epidemiol Biomarkers Prev, 2006, 15(2): 266-271. doi: 10.1158/1055-9965.EPI-05-0647
[5]	Hartmann EC, Boettcher MI, Bolt HM, et al. N-Acetyl-S-(1- carbamoyl-2-hydroxy-ethyl)-L-cysteine (iso-GAMA) a further product of human metabolism of acrylamide: comparison with the simultaneously excreted other mercaptuic acids[J]. Arch Toxicol, 2009, 83(7): 731-734. doi: 10.1007/s00204-008-0369-8
[6]	Park HR, Kim MS, Kim SJ, et al. Acrylamide induces cell death in neural progenitor cells and impairs hippocampal neurogenesis[J]. Toxicol Lett, 2010, 193(1): 86-93. doi: 10.1016/j.toxlet.2009.12.015
[7]	Von Tungeln LS, Doerge DR, Gamboa da Costa G, et al. Tumorigenicity of acrylamide and its metabolite glycidamide in the neonatal mouse bioassay[J]. Int J Cancer, 2012, 131(9): 2008-2015. doi: 10.1002/ijc.27493
[8]	Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1): 15-20. doi: 10.1016/j.cell.2004.12.035
[9]	Ambros V. The functions of animal microRNAs[J]. Nature, 2004, 431(7006): 350-355. doi: 10.1038/nature02871
[10]	Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells[J]. Nat Methods, 2007, 4(9): 721-726. doi: 10.1038/nmeth1079
[11]	Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers[J]. Nature, 2005, 435(7043): 834-838. doi: 10.1038/nature03702
[12]	Meng F, Henson R, Wehbe-Janek H, et al. MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer[J]. Gastroenterology, 2007, 133(2): 647-658. doi: 10.1053/j.gastro.2007.05.022
[13]	Zhang J, Jiao J, Cermelli S, et al. miR-21 Inhibition Reduces Liver Fibrosis and Prevents Tumor Development by Inducing Apoptosis of CD24+ Progenitor Cells[J]. Cancer Res, 2015, 75(9): 1859-1867. doi: 10.1158/0008-5472.CAN-14-1254
[14]	Caserta E, Egriboz O, Wang H, et al. Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo[J]. Genes Dev, 2015, 29(16): 1707-1720. doi: 10.1101/gad.262568.115
[15]	Stavarache MA, Musatov S, McGill M, et al. The tumor suppressor PTEN regulates motor responses to striatal dopamine in normal and Parkinsonian animals[J]. Neurobiol Dis, 2015, 82: 487-494. doi: 10.1016/j.nbd.2015.07.013
[16]	Chalhoub N, Baker SJ. PTEN and the PI3-kinase pathway in cancer[J]. Annu Rev Pathol, 2009, 4: 127-150. doi: 10.1146/annurev.pathol.4.110807.092311
[17]	Ciuffreda L, Falcone I, Incani UC, et al. PTEN expression and function in adult cancer stem cells and prospects for therapeutic targeting[J]. Adv Biol Regul, 2014, 56: 66-80. doi: 10.1016/j.jbior.2014.07.002
[18]	de Araujo WM, Robbs BK, Bastos LG, et al. PTEN Overexpression Cooperates With Lithium to Reduce the Malignancy and to Increase Cell Death by Apoptosis via PI3K/Akt Suppression in Colorectal Cancer Cells[J]. J Cell Biochem, 2016, 117(2): 458-469. doi: 10.1002/jcb.25294
[19]	Shan X, Li Y, Meng X, et al. Curcumin and (-)-epigallocatechin-3-gallate attenuate acrylamide-induced proliferation in HepG2 cells[J]. Food Chem Toxicol, 2014, 66: 194-202. doi: 10.1016/j.fct.2014.01.046
[20]	Go H, Jang JY, Kim PJ, et al. MicroRNA-21 plays an oncogenic role by targeting FOXO1 and activating the PI3K/ AKT pathway in diffuse large B-cell lymphoma[J]. Oncotarget, 2015, 6(17): 15035-15049. http://cn.bing.com/academic/profile?id=6b7a74d69e3775c84c723425a3ff7ade&encoded=0&v=paper_preview&mkt=zh-cn
[21]	Yan-nan B, Zhao-yan Y, Li-xi L, et al. MicroRNA-21 accelerates hepatocyte proliferation in vitro via PI3K/Akt signaling by targeting PTEN[J]. Biochem Biophys Res Commun, 2014, 443(3): 802-807. doi: 10.1016/j.bbrc.2013.12.047
[22]	Kranthi KKT, Ganugula R, Gade DR, et al. Gedunin abrogates aldose reductase, PI3K/Akt/mToR, and NF-κB signaling pathways to inhibit angiogenesis in a hamster model of oral carcinogenesis[J]. Tumour Biol, 2016, 37: 1-11. http://cn.bing.com/academic/profile?id=a05523ff4810afafc554650d3048fe24&encoded=0&v=paper_preview&mkt=zh-cn
[23]	Miyagawa S, Sato M, Sudo T, et al. Unique roles of estrogendependent Pten control in epithelial cell homeostasis of mouse vagina[J]. Oncogene, 2015, 34(8): 1035-1043. doi: 10.1038/onc.2014.62
[24]	Parker VER, Parker VE, Knox RG, Zhang Q, et al. Phosphoinositide 3-kinase-related overgrowth: cellular phenotype and future therapeutic options[J]. Lancet, 2015, 385(Suppl 1): S77 PMID: 26312899. http://www.researchgate.net/publication/276385781_Phosphoinositide_3-kinase-related_overgrowth_cellular_phenotype_and_future_therapeutic_options
[25]	Bradford BU, Kono H, Isayama F, et al. Cytochrome P450 CYP2E1, but not nicotinamide adenine dinucleotide phosphate oxidase, is required for ethanol-induced oxidative DNA damage in rodent liver[J]. Hepatology, 2005, 41(2): 336-344. doi: 10.1002/(ISSN)1527-3350
[26]	Brandon-Warner E, Sugg JA, Schrum LW, et al. Silibinin inhibits ethanol metabolism and ethanol-dependent cell proliferation in an in vitro model of hepatocellular carcinoma[J]. Cancer Lett, 2010, 291(1): 120-129. doi: 10.1016/j.canlet.2009.10.004
[27]	Ning BF, Ding J, Liu J, et al. Hepatocyte nuclear factor 4α- nuclear factor-κB feedback circuit modulates liver cancer progression[J]. Hepatology, 2014, 60(5): 1607-1619. doi: 10.1002/hep.v60.5
[28]	Liu H, Lou G, Li C, et al. HBx inhibits CYP2E1 gene expression via downregulating HNF4α in human hepatoma cells[J]. PLoS One, 2014, 9(9): e107913-e107913. doi: 10.1371/journal.pone.0107913
[29]	Beland FA, Mellick PW, Olson GR, et al. Carcinogenicity of acrylamide in B6C3F₁ mice and F344/N rats from a 2-year drinking water exposure[J]. Food Chem Toxicol, 2013, 51: 149-159. doi: 10.1016/j.fct.2012.09.017
[30]	Authority EFS. Results on acrylamide levels in food from monitoring years 2007-2009 and Exposure assessment[J]. Efsa Journal 2011, 9(4): 2133. doi: 10.2903/j.efsa.2011.2133
[31]	Sen A, Ozgun O, Arinç E, et al. Diverse action of acrylamide on cytochrome P450 and glutathione S-transferase isozyme activities, mRNA levels and protein levels in human hepatocarcinoma cells[J]. Cell Biol Toxicol, 2012, 28(3): 175-186. doi: 10.1007/s10565-012-9214-1
[32]	Fiala M. Curcumin and omega-3 fatty acids enhance NK cellinduced apoptosis of pancreatic cancer cells but curcumin inhibits interferon-γ production: benefits of omega-3 with curcumin against cancer[J]. Molecules, 2015, 20(2): 3020- 3026. doi: 10.3390/molecules20023020
[33]	Xu Y, Zhang J, Han J, et al. Curcumin inhibits tumor proliferation induced by neutrophil elastase through the upregulation of α1-antitrypsin in lung cancer[J]. Mol Oncol, 2012, 6(4): 405-417. doi: 10.1016/j.molonc.2012.03.005
[34]	Toden S, Okugawa Y, Jascur T, et al. Curcumin mediates chemosensitization to 5-fluorouracil through miRNA-induced suppression of epithelial-to-mesenchymal transition in chemoresistant colorectal cancer[J]. Carcinogenesis, 2015, 36(3): 355-367. doi: 10.1093/carcin/bgv006
[35]	Huang YS, Hsieh TJ, Lu CY. Simple analytical strategy for MALDI-TOF-MS and nanoUPLC-MS/MS: quantitating curcumin in food condiments and dietary supplements and screening of acrylamide-induced ROS protein indicators reduced by curcumin[J]. Food Chem, 2015, 174: 571-576. doi: 10.1016/j.foodchem.2014.11.115
[36]	James MI, Iwuji C, Irving G, et al. Curcumin inhibits cancer stem cell phenotypes in ex vivo models of colorectal liver metastases, and is clinically safe and tolerable in combination with FOLFOX chemotherapy[J]. Cancer Lett, 2015, 364(2): 135-141. doi: 10.1016/j.canlet.2015.05.005
[37]	Kantara C, O'Connell M, Sarkar S, et al. Curcumin promotes autophagic survival of a subset of colon cancer stem cells, which are ablated by DCLK1-siRNA[J]. Cancer Res, 2014, 74(9): 2487-2498. doi: 10.1158/0008-5472.CAN-13-3536
[38]	Ireson C, Orr S, Jones DJ, et al. Characterization of metabolites of the chemopreventive agent curcumin in human and rat hepatocytes and in the rat in vivo, and evaluation of their ability to inhibit phorbol ester-induced prostaglandin E2 production[J]. Cancer Res, 2001, 61(3): 1058-1064. http://cn.bing.com/academic/profile?id=e75c60ed8434cf4401e155bbbfa787c9&encoded=0&v=paper_preview&mkt=zh-cn

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1.	Boda VK, Yasmen N, Jiang J, et al. Pathophysiological significance and modulation of the transient receptor potential canonical 3 ion channel. Med Res Rev, 2024, 44(6): 2510-2544. DOI:10.1002/med.22048
2.	Agrawal K, Asthana S, Kumar D. Role of Oxidative Stress in Metabolic Reprogramming of Brain Cancer. Cancers (Basel), 2023, 15(20): 4920. DOI:10.3390/cancers15204920
3.	Zhou Y, Pereira G, Tang Y, et al. 3D Porous Scaffold-Based High-Throughput Platform for Cancer Drug Screening. Pharmaceutics, 2023, 15(6): 1691. DOI:10.3390/pharmaceutics15061691
4.	Safa AR. Drug and apoptosis resistance in cancer stem cells: a puzzle with many pieces. Cancer Drug Resist, 2022, 5(4): 850-872. DOI:10.20517/cdr.2022.20
5.	Gal O, Betzer O, Rousso-Noori L, et al. Antibody Delivery into the Brain by Radiosensitizer Nanoparticles for Targeted Glioblastoma Therapy. J Nanotheranostics, 2022, 3(4): 177-188. DOI:10.3390/jnt3040012
6.	Scioli MG, Terriaca S, Fiorelli E, et al. Extracellular Vesicles and Cancer Stem Cells in Tumor Progression: New Therapeutic Perspectives. Int J Mol Sci, 2021, 22(19): 10572. DOI:10.3390/ijms221910572
7.	Keyvani-Ghamsari S, Khorsandi K, Rasul A, et al. Current understanding of epigenetics mechanism as a novel target in reducing cancer stem cells resistance. Clin Epigenetics, 2021, 13(1): 120. DOI:10.1186/s13148-021-01107-4
8.	Safa AR. Resistance to drugs and cell death in cancer stem cells (CSCs). J Transl Sci, 2020, 6(3): 341. DOI:10.15761/jts.1000341
9.	Chandimali N, Koh H, Kim J, et al. BRM270 targets cancer stem cells and augments chemo-sensitivity in cancer. Oncol Lett, 2020, 20(4): 103. DOI:10.3892/ol.2020.11964
10.	Mukherjee S. Quiescent stem cell marker genes in glioma gene networks are sufficient to distinguish between normal and glioblastoma (GBM) samples. Sci Rep, 2020, 10(1): 10937. DOI:10.1038/s41598-020-67753-5
11.	Zhou JJ, Xiao Y, Li H, et al. Overexpression of Malic Enzyme 2 Indicates Pathological and Clinical Significance in Oral Squamous Cell Carcinoma. Int J Med Sci, 2020, 17(6): 799-806. DOI:10.7150/ijms.43832
12.	Sun Z, Wang L, Zhou Y, et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol, 2020, 40(5): 767-784. DOI:10.1007/s10571-019-00771-8
13.	Zhang Q, Xu B, Chen J, et al. Clinical significance of CD133 and Nestin in astrocytic tumor: The correlation with pathological grade and survival. J Clin Lab Anal, 2020, 34(3): e23082. DOI:10.1002/jcla.23082
14.	Megías J, Martínez A, San-Miguel T, et al. Pam3CSK4, a TLR2 ligand, induces differentiation of glioblastoma stem cells and confers susceptibility to temozolomide. Invest New Drugs, 2020, 38(2): 299-310. DOI:10.1007/s10637-019-00788-2
15.	Li Z, Chen Y, An T, et al. Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway. J Exp Clin Cancer Res, 2019, 38(1): 139. DOI:10.1186/s13046-019-1134-y
16.	Ghosh D, Nandi S, Bhattacharjee S. Combination therapy to checkmate Glioblastoma: clinical challenges and advances. Clin Transl Med, 2018, 7(1): 33. DOI:10.1186/s40169-018-0211-8
17.	Grande S, Palma A, Ricci-Vitiani L, et al. Metabolic Heterogeneity Evidenced by MRS among Patient-Derived Glioblastoma Multiforme Stem-Like Cells Accounts for Cell Clustering and Different Responses to Drugs. Stem Cells Int, 2018, 2018: 3292704. DOI:10.1155/2018/3292704
18.	Zuccarini M, Giuliani P, Ziberi S, et al. The Role of Wnt Signal in Glioblastoma Development and Progression: A Possible New Pharmacological Target for the Therapy of This Tumor. Genes (Basel), 2018, 9(2): 105. DOI:10.3390/genes9020105
19.	Bhere D, Tamura K, Wakimoto H, et al. microRNA-7 upregulates death receptor 5 and primes resistant brain tumors to caspase-mediated apoptosis. Neuro Oncol, 2018, 20(2): 215-224. DOI:10.1093/neuonc/nox138
20.	Lee S, Kwon MC, Jang JP, et al. The ginsenoside metabolite compound K inhibits growth, migration and stemness of glioblastoma cells. Int J Oncol, 2017, 51(2): 414-424. DOI:10.3892/ijo.2017.4054
21.	Jovčevska I, Zupanec N, Urlep Ž, et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget, 2017, 8(27): 44141-44158. DOI:10.18632/oncotarget.17390
22.	Hiramatsu H, Kobayashi K, Kobayashi K, et al. The role of the SWI/SNF chromatin remodeling complex in maintaining the stemness of glioma initiating cells. Sci Rep, 2017, 7(1): 889. DOI:10.1038/s41598-017-00982-3
23.	Zheng X, Pang B, Gu G, et al. Melatonin Inhibits Glioblastoma Stem-like cells through Suppression of EZH2-NOTCH1 Signaling Axis. Int J Biol Sci, 2017, 13(2): 245-253. DOI:10.7150/ijbs.16818
24.	Bijangi-Vishehsaraei K, Reza Saadatzadeh M, Wang H, et al. Sulforaphane suppresses the growth of glioblastoma cells, glioblastoma stem cell-like spheroids, and tumor xenografts through multiple cell signaling pathways. J Neurosurg, 2017, 127(6): 1219-1230. DOI:10.3171/2016.8.JNS161197
25.	Majewska E, Szeliga M. AKT/GSK3β Signaling in Glioblastoma. Neurochem Res, 2017, 42(3): 918-924. DOI:10.1007/s11064-016-2044-4
26.	Kanabur P, Guo S, Simonds GR, et al. Patient-derived glioblastoma stem cells respond differentially to targeted therapies. Oncotarget, 2016, 7(52): 86406-86419. DOI:10.18632/oncotarget.13415
27.	Wang K, Kievit FM, Erickson AE, et al. Culture on 3D Chitosan-Hyaluronic Acid Scaffolds Enhances Stem Cell Marker Expression and Drug Resistance in Human Glioblastoma Cancer Stem Cells. Adv Healthc Mater, 2016, 5(24): 3173-3181. DOI:10.1002/adhm.201600684

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Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression