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  • ISSN 1674-8301
  • CN 32-1810/R
Volume 29 Issue 5
Aug.  2015
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Xianjin Ke, Qian Li, Li Xu, Ying Zhang, Dongmei Li, Jianhua Ma, Xiaoming Mao. Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury[J]. The Journal of Biomedical Research, 2015, 29(5): 380-389. DOI: 10.7555/JBR.29.20140076
Citation: Xianjin Ke, Qian Li, Li Xu, Ying Zhang, Dongmei Li, Jianhua Ma, Xiaoming Mao. Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury[J]. The Journal of Biomedical Research, 2015, 29(5): 380-389. DOI: 10.7555/JBR.29.20140076
shu

Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury

  • 1.

    Department of Neurology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China

  • 2.

    Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China

Funds: 

Jiangsu Planned Projects for Postdoctoral Research Funds, Nanjing Medical Technology Development Project (No. ZKX08014), Nanjing Medical Science and Technique Development Foundation, National Natural Science Foundation of China (No. 81200594)

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  • Received Date: May 15, 2014
  • Revised Date: September 30, 2014
    Graphical Abstract
    • Abstract
  • Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been developed as a new method of treating diseases of the peripheral nervous system. While netrin-1 is a critical molecule for axonal path finding and nerve growth, it may also affect vascular network formation. Here, we investigated the effect of transplanting BMSCs that produce netrin-1 in a rat model of sciatic nerve crush injury. We introduced a sciatic nerve crush injury, and then injected 16106 BMSCs infected by a recombinant adenovirus expressing netrin-1 Ad5-Netrin-1-EGFP or culture medium into the injured part in the next day. At day 7, 14 and 28 after injection, we measured motor nerve conduction and detected mRNA expressions of netrin-1 receptors UNC5B and Deleted in Colorectal Cancer (DCC), and neurotrophic factors brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) by real-time PCR. We also detected protein expressions of BDNF and NGF by Western blotting assays and examined BMSCs that incorporated into myelin and vascellum. The results showed that BMSCs infected by Ad5-Netrin- 1-EGFP significantly improved the function of the sciatic nerve, and led to increased expression of BDNF and NGF (P,0.05). Moreover, 28 days after injury, more Schwann cells were found in BMSCs infected by Ad5- Netrin-1-EGFP compared to control BMSCs. In conclusion, transplantation of BMSCs that produce netrin-1 improved the function of the sciatic nerve after injury. This method may be a new treatment of nerve injury.
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    1. Liu Y, Zhang X, Xiao C, et al. Engineered hydrogels for peripheral nerve repair. Mater Today Bio, 2023, 20: 100668. DOI:10.1016/j.mtbio.2023.100668
    2. Zhou H, He Y, Xiong W, et al. MSC based gene delivery methods and strategies improve the therapeutic efficacy of neurological diseases. Bioact Mater, 2022, 23: 409-437. DOI:10.1016/j.bioactmat.2022.11.007
    3. Wang H, Wang F, Wang Y, et al. Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury. J Musculoskelet Neuronal Interact, 2022, 22(4): 546-561.
    4. Sun Q, Li G, Liu D, et al. Peripheral nerves in the tibial subchondral bone: the role of pain and homeostasis in osteoarthritis. Bone Joint Res, 2022, 11(7): 439-452. DOI:10.1302/2046-3758.117.BJR-2021-0355.R1
    5. Chen JT, Schmidt L, Schürger C, et al. Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury. Int J Mol Sci, 2021, 22(18): 10090. DOI:10.3390/ijms221810090
    6. Wan QQ, Qin WP, Ma YX, et al. Crosstalk between Bone and Nerves within Bone. Adv Sci (Weinh), 2021, 8(7): 2003390. DOI:10.1002/advs.202003390
    7. Ding S, Guo X, Zhu L, et al. Macrophage-derived netrin-1 contributes to endometriosis-associated pain. Ann Transl Med, 2021, 9(1): 29. DOI:10.21037/atm-20-2161
    8. Gao R, Peng X, Perry C, et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest, 2021, 131(1): e136542. DOI:10.1172/JCI136542
    9. Dun XP, Parkinson DB. Classic axon guidance molecules control correct nerve bridge tissue formation and precise axon regeneration. Neural Regen Res, 2020, 15(1): 6-9. DOI:10.4103/1673-5374.264441
    10. Karamzadeh T, Alipour H, Shahriari-Namadi M, et al. Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae. AIMS Genet, 2019, 6(3): 46-54. DOI:10.3934/genet.2019.3.46
    11. Ko SY, Price JT, Blatch GL, et al. Netrin-1-like-immunoreactivity Coexpresses With DCC and Has a Differential Level in the Myenteric Cholinergic and Nitrergic Neurons of the Adult Mouse Colon. J Histochem Cytochem, 2019, 67(5): 335-349. DOI:10.1369/0022155418819821
    12. Huang CW, Huang WC, Qiu X, et al. The Differentiation Stage of Transplanted Stem Cells Modulates Nerve Regeneration. Sci Rep, 2017, 7(1): 17401. DOI:10.1038/s41598-017-17043-4
    13. Dun XP, Parkinson DB. Role of Netrin-1 Signaling in Nerve Regeneration. Int J Mol Sci, 2017, 18(3): 491. DOI:10.3390/ijms18030491
    14. Prieto CP, Ortiz MC, Villanueva A, et al. Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC). Stem Cell Res Ther, 2017, 8(1): 43. DOI:10.1186/s13287-017-0494-5
    15. Lee SS, Lee SJ, Lee SH, et al. Netrin-1-Induced Stem Cell Bioactivity Contributes to the Regeneration of Injured Tissues via the Lipid Raft-Dependent Integrin α6β4 Signaling Pathway. Sci Rep, 2016, 6: 37526. DOI:10.1038/srep37526
    16. Li S, Hua Y, Jin J, et al. Association of genetic variants in lncRNA H19 with risk of colorectal cancer in a Chinese population. Oncotarget, 2016, 7(18): 25470-7. DOI:10.18632/oncotarget.8330

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