4.6

CiteScore

2.2

Impact Factor
  • ISSN 1674-8301
  • CN 32-1810/R
Lan Lin, Zhiyi Qiang, Kaiao Chen, Ying Huo, Wei Liu, Jian Yang. DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation[J]. The Journal of Biomedical Research. DOI: 10.7555/JBR.38.20240069
Citation: Lan Lin, Zhiyi Qiang, Kaiao Chen, Ying Huo, Wei Liu, Jian Yang. DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation[J]. The Journal of Biomedical Research. DOI: 10.7555/JBR.38.20240069

DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation

  • Previous studies have shown that differentiated embryo-chondrocyte expressed gene 1 (DEC1) promotes osteoblast osteogenesis. To investigate the role of DEC1 in postmenopausal osteoporosis (PMOP), we utilized the two types (DEC1+/+, DEC1−/−) mice to establish an ovariectomy (OVX) model and found that the bone loss in DEC1−/− OVX mice were much less than that in DEC1+/+ OVX mice. The expression levels of RUNX2 and OSX significantly increased in DEC1−/− OVX mice compared with those in DEC1+/+ OVX mice. Whereas, NFATc1, c-Fos, CTSK and RANKL/OPG significantly decreased in DEC1−/− OVX mice compared with those in DEC1+/+ OVX mice. Likewise, DEC1 deficiency suppressed IL-6 and IL-1β. Further study showed Runx2, Osx, Alp, and Ocn significantly increased in DEC1−/− OVX BMSCs compared with those in DEC1+/+ OVX BMSCs. And the mRNA levels of IL-1β, IL-6, Tnf-α and Ifn-γ increased significantly in DEC1+/+ OVX BMMs compared with those in DEC1+/+ sham BMMs, but not in DEC1−/− OVX BMMs compared with those in DEC1−/− sham BMMs. Furthermore, the p-IκBα and p-P65 significantly increased in DEC1+/+ OVX BMMs compared with those in DEC1+/+ sham BMMs, but did not increase in DEC1−/− OVX BMMs compared with those in DEC1−/− sham BMMs. Taken together, DEC1 deficiency inhibits the NF-κB pathway induced by OVX, thereby decreasing cytokines, and subsequently, inhibits the decrease of osteogenesis and the increase of osteoclastogenesis caused by OVX. The findings provide a novel understanding of postmenopausal osteoporosis development, which offers potential avenues for the intervention strategies.
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