EphA3 deficiency in the hypothalamus promotes high-fat diet-induced obesity in mice

Jubiao Zhang; Yang Chen; Lihong Yan; Xin Zhang; Xiaoyan Zheng; Junxia Qi; Fen Yang; Juxue Li

doi:10.7555/JBR.36.20220168

Volume 37 Issue 3

May 2023

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Article Navigation > The Journal of Biomedical Research > 2023 > 37(3): 179-193

Jubiao Zhang, Yang Chen, Lihong Yan, Xin Zhang, Xiaoyan Zheng, Junxia Qi, Fen Yang, Juxue Li. EphA3 deficiency in the hypothalamus promotes high-fat diet-induced obesity in mice[J]. The Journal of Biomedical Research, 2023, 37(3): 179-193. doi: 10.7555/JBR.36.20220168

Citation:

Jubiao Zhang, Yang Chen, Lihong Yan, Xin Zhang, Xiaoyan Zheng, Junxia Qi, Fen Yang, Juxue Li. EphA3 deficiency in the hypothalamus promotes high-fat diet-induced obesity in mice[J]. The Journal of Biomedical Research, 2023, 37(3): 179-193. doi: 10.7555/JBR.36.20220168

Citation:

Jubiao Zhang, Yang Chen, Lihong Yan, Xin Zhang, Xiaoyan Zheng, Junxia Qi, Fen Yang, Juxue Li. EphA3 deficiency in the hypothalamus promotes high-fat diet-induced obesity in mice[J]. The Journal of Biomedical Research, 2023, 37(3): 179-193. doi: 10.7555/JBR.36.20220168

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EphA3 deficiency in the hypothalamus promotes high-fat diet-induced obesity in mice

doi: 10.7555/JBR.36.20220168

Jubiao Zhang^{1, 2, 3, △},
Yang Chen^{1, 2, 3, △},
Lihong Yan^{1, 2, 3},
Xin Zhang^{1, 2, 3},
Xiaoyan Zheng^{1, 2},
Junxia Qi^{1, 2},
Fen Yang^{1, 2},
Juxue Li^{1, 2, 3, 4
,
,}

1.
State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China
2.
Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China
3.
The Affiliated Eye Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
4.
The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, China

Funds: This work was supported by National Natural Science Foundation of China (Grants No. 82070872, 92049118, and 81570774), the Jiangsu Province's Innovation Personal as well as Innovative and Entrepreneurial Team of Jiangsu Province (Grant No. JSSCTD2021), the National Key Research and Development Program of China (Grant No. 2018YFC1003504), the Junior Thousand Talents Program of China, the NJMU startup fund.

More Information

Corresponding author: Juxue Li, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 101 Longmian Avenue, Nanjing, Jiangsu 211166, China. Tel: +86-25-86869392, E-mail: lijuxue@njmu.edu.cn
Received: 2022-07-30
Revised: 2022-10-24
Accepted: 2022-11-06

Published: 2022-11-28

Issue Date: 2023-05-28

Abstract

Abstract

Erythropoietin-producing hepatocellular carcinoma A3 (EphA3) is a member of the largest subfamily of tyrosine kinase receptors—Eph receptors. Previous studies have shown that EphA3 is associated with tissue development. Recently, we have found that the expression of EphA3 is elevated in the hypothalamus of mice with diet-induced obesity (DIO). However, the role of EphA3 in hypothalamic-controlled energy metabolism remains unclear. In the current study, we demonstrated that the deletion of EphA3 in the hypothalamus by CRISPR/Cas9-mediated gene editing promotes obesity in male mice with high-fat diet feeding rather than those with normal chow diet feeding. Moreover, the deletion of hypothalamic EphA3 promotes high-fat DIO by increasing food intake and reducing energy expenditure. Knockdown of EphA3 leads to smaller intracellular vesicles in GT1-7 cells. The current study reveals that hypothalamic EphA3 plays important roles in promoting DIO.
- EphA3,
- hypothalamus,
- metabolism,
- CRISPR-Cas9,
- obesity

CLC number: R589, Document code: A
The authors reported no conflict of interests.
Δ These authors contributed equally to this work.

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References(30)

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