• ISSN 1674-8301
  • CN 32-1810/R
Volume 36 Issue 4
Jul.  2022
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Article Contents
Xiangdong Hua, Yan Zhang, Juan Xu, Lu Xu, Yaqian Shi, Dazhen Yang, Xiaoyan Gu, Sumin Wang, Xuemei Jia, Feng Xu, Jie Chen, Xiaoyan Ying. Peptidome analysis of human intrauterine adhesion tissues and the identification of antifibrotic peptide[J]. The Journal of Biomedical Research, 2022, 36(4): 280-296. doi: 10.7555/JBR.36.20220059
Citation: Xiangdong Hua, Yan Zhang, Juan Xu, Lu Xu, Yaqian Shi, Dazhen Yang, Xiaoyan Gu, Sumin Wang, Xuemei Jia, Feng Xu, Jie Chen, Xiaoyan Ying. Peptidome analysis of human intrauterine adhesion tissues and the identification of antifibrotic peptide[J]. The Journal of Biomedical Research, 2022, 36(4): 280-296. doi: 10.7555/JBR.36.20220059

Peptidome analysis of human intrauterine adhesion tissues and the identification of antifibrotic peptide

doi: 10.7555/JBR.36.20220059
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  • Corresponding author: Xiangdong Hua, Department of Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123 Mochou Rd, Nanjing, Jiangsu 210004, China. Tel: +86-25-52226961, E-mail: hxd_82@126.com; Xiaoyan Ying, Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Rd, Nanjing, Jiangsu 210011, China. Tel: +86-25-58509900, E-mail: xiaoyanying_efy@163.com
  • Received: 2022-03-20
  • Revised: 2022-06-19
  • Accepted: 2022-06-27
  • Published: 2022-07-28
  • Issue Date: 2022-07-28
  • Intrauterine adhesion (IUA) is a common clinical endometrial disease, which can severely damage the fertility and quality of life in women. This study aims to find the differentially expressed endogenous peptides and their possible roles in IUA. Liquid chromatography-mass spectrometry was used to identify the peptidomic profiling of IUA tissues, and the differentially expressed peptides were screened out. Using real-time quantitative PCR, Western blotting, and immunocytochemistry staining, the function of six endogenous peptides was verified in vitro. It was found that peptide 6 (T6) (peptide sequence: TFGGAPGFPLGSPLSSVFPR) could inhibit the expression of TGF-β1-induced cell fibrosis in human endometrial stromal cell line and primary human endometrial stromal cell at a concentration of 50 μmol/L. This study provides new targets for further clarifying the formation and prevention of IUA.

     

  • CLC number: R711.4, Document code: A
    The authors reported no conflict of interests.
    △These authors contributed equally to this work.
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