Lei Wang, Sixin Sun, Lei Yang, Chun Lu, Xiaojian Cao. Effects of leptin on femoral fracture in rats[J]. The Journal of Biomedical Research, 2018, 32(2): 130-135. DOI: 10.7555/JBR.31.20160077
Citation:
Lei Wang, Sixin Sun, Lei Yang, Chun Lu, Xiaojian Cao. Effects of leptin on femoral fracture in rats[J]. The Journal of Biomedical Research, 2018, 32(2): 130-135. DOI: 10.7555/JBR.31.20160077
Lei Wang, Sixin Sun, Lei Yang, Chun Lu, Xiaojian Cao. Effects of leptin on femoral fracture in rats[J]. The Journal of Biomedical Research, 2018, 32(2): 130-135. DOI: 10.7555/JBR.31.20160077
Citation:
Lei Wang, Sixin Sun, Lei Yang, Chun Lu, Xiaojian Cao. Effects of leptin on femoral fracture in rats[J]. The Journal of Biomedical Research, 2018, 32(2): 130-135. DOI: 10.7555/JBR.31.20160077
In this study, our objective was to evaluate effects of leptin on fracture healing in rats. Seventy two male SpragueDawley (SD) rats were randomized into 3 groups. Standardized femoral fractures were created in all the rats. Group A
was treated with 1 mL normal saline (NS), group B with 0.3 μg/kg leptin in 1 mL NS, and group C with 0.5 μg/kg
leptin in 1 mL NS for 2 weeks intraperitoneally. Each group was divided into three subgroups including 8 rats for
evaluation at 2, 4 and 8 weeks. Radiological evaluation showed that callus formation of group B and C was all
significantly higher than group A at 8 weeks (P = 0.04 and P = 0.013, respectively). There was no statistically
significant difference in fracture healing between group B and group C at 8 weeks (P = 0.197). Histological
evaluation revealed fracture healing of group B and C was better than group A at 4 weeks (P = 0.01 and P = 0.002,
respectively) and 8 weeks (P = 0.008 and P = 0.003, respectively). Micro-computed tomography (Micro-CT) analysis
demonstrated that greater amounts of bony callus and evidence of bone fusion were observed in group B and C at 4
weeks (P = 0.02 and P = 0.04, respectively) and 8 weeks (P = 0.005 and P = 0.001, respectively) compared to group
A. Group C also had better fracture healing than group B at 8 weeks (P = 0.01). In conclusion, leptin has a positive
effect on rat femoral fracture healing.
De la Cruz-Color L, Dominguez-Rosales JA, Maldonado-González M, et al. Evidence That Peripheral Leptin Resistance in Omental Adipose Tissue and Liver Correlates with MASLD in Humans. Int J Mol Sci, 2024, 25(12): 6420.
DOI:10.3390/ijms25126420
2.
Casado ME, Collado-Pérez R, Frago LM, et al. Recent Advances in the Knowledge of the Mechanisms of Leptin Physiology and Actions in Neurological and Metabolic Pathologies. Int J Mol Sci, 2023, 24(2): 1422.
DOI:10.3390/ijms24021422
3.
Chenouard V, Remy S, Tesson L, et al. Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models. Front Genet, 2021, 12: 615491.
DOI:10.3389/fgene.2021.615491
4.
Lin SY, Yang CP, Wang YY, et al. Interleukin-4 Improves Metabolic Abnormalities in Leptin-Deficient and High-Fat Diet Mice. Int J Mol Sci, 2020, 21(12): 4451.
DOI:10.3390/ijms21124451