Guanhua Su, Kun Liu, Yan Wang, Jue Wang, Xiaowei Li, Wenzhu Li, Yuhua Liao, Zhaohui Wang. Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy[J]. The Journal of Biomedical Research, 2011, 25(2): 120-127. DOI: 10.1016/S1674-8301(11)60015-8
Citation:
Guanhua Su, Kun Liu, Yan Wang, Jue Wang, Xiaowei Li, Wenzhu Li, Yuhua Liao, Zhaohui Wang. Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy[J]. The Journal of Biomedical Research, 2011, 25(2): 120-127. DOI: 10.1016/S1674-8301(11)60015-8
Guanhua Su, Kun Liu, Yan Wang, Jue Wang, Xiaowei Li, Wenzhu Li, Yuhua Liao, Zhaohui Wang. Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy[J]. The Journal of Biomedical Research, 2011, 25(2): 120-127. DOI: 10.1016/S1674-8301(11)60015-8
Citation:
Guanhua Su, Kun Liu, Yan Wang, Jue Wang, Xiaowei Li, Wenzhu Li, Yuhua Liao, Zhaohui Wang. Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy[J]. The Journal of Biomedical Research, 2011, 25(2): 120-127. DOI: 10.1016/S1674-8301(11)60015-8
Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, Hubei, China
2.
Division of Clinics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
Funds:
This study was supported by CGICC Medical Science Research Sup-porting Program (No.08010022) and National Program on Key Basic Research Project of China (No. 2007CB512000, Sub-Project No. 2007CB512005).
Fibrinogen-like protein 2 (fgl2), a novel prothrombinase, is involved in microthrombosis. We examined fgl2 expression in the glomerular and tubulointerstitial capillaries and its correlation with microthromsis in rats with streptozocin-induced type 2 diabetic nephropathy. Our RT-PCR and immunoblotting analysis showed that fgl2 mRNA and protein levels were increased in microvascular endothelial cells of the glomeruli and renal interstitia at week 19 and became significantly elevated with the development of diabetic nephropathy (P < 0.01). Fgl2 was not or only weakly expressed in the renal tissues of normal rats. Furthermore, a direct significant correlation (r = 0.543, P < 0.01) was found between fgl2 expression and microthrombotic capillaries in the renal tissues. Enzyme linked immunosorbent assays (ELISA) additionally showed that circulating TNF-α levels in rats with type 2 diabetes were significantly elevated and closely correlated with fgl2 expression (r = 0.871, P < 0.01). Our results suggest that fgl2 may activate renal microthrombosis, thus contributing to glomerular hypertension and renal ischemia.
Authors and Reviewers
DownLoad: