Inactivation?of?Staphylococcus?aureus?and?Enterococcus?faecalis?by?a?direct-current,?cold?atmospheric-pressure?air?plasma?microjet

Ye?Tian; Peng?Sun; Haiyan?Wu; Na?Bai; Ruixue?Wang; Weidong?Zhu; Jue?Zhang; Fuxiang??Liu

doi:10.1016/S1674-8301(10)60037-1

Volume 24 Issue 4

Jun. 2010

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The Journal of Biomedical Research > 2010 > 24(4): 264-269. > DOI: 10.1016/S1674-8301(10)60037-1

Ye?Tian, Peng?Sun, Haiyan?Wu, Na?Bai, Ruixue?Wang, Weidong?Zhu, Jue?Zhang, Fuxiang??Liu. Inactivation?of?Staphylococcus?aureus?and?Enterococcus?faecalis?by?a?direct-current,?cold?atmospheric-pressure?air?plasma?microjet[J]. The Journal of Biomedical Research, 2010, 24(4): 264-269. DOI: 10.1016/S1674-8301(10)60037-1

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Inactivation?of?Staphylococcus?aureus?and?Enterococcus?faecalis?by?a?direct-current,?cold?atmospheric-pressure?air?plasma?microjet

1.
West?China?College?of?Stomatology,?Sichuan?University,?Chengdu?610041,?Sichuan?province,?China
2.
Department?of?Biomedical?Engineering,?College?of?Engineering,?Peking?University,?Beijing?100871,?China
3.
Academy?for?Advanced?Interdisciplinary?Studies,?Peking?University,?Beijing?100871,?China
4.
Department of Applied Science and Technology, Saint Peter's College, New Jersey 07306, USA
5.
Department?of?Biomedical?Engineering,?College?of?Engineering,?Peking?University,?Beijing?100871,?China?/Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
6.
West?China?College?of?Stomatology,?Sichuan?University,?Chengdu?610041,?Sichuan?province,?China/Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China

Funds:

This study was supported by Bioelectrics Inc.(USA),Peking University Biomed-X Foundation and China International Science and Technology Cooperation(No.2008KR1330)

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Received Date: March 28, 2010

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Abstract

Abstract

Objective:?A?direct-current,?cold?atmospheric-pressure?air?plasma?microjet?(PMJ)?was?performed?to?inactivate?Staphylococcus?aureus?(S.?aureus)?and?Enterococcus?faecalis?(E.?faecalis)?in?air.?The?process?of?sterilization?and?morphology?of?bacteria?was?observed.?We?wish?to?know?the?possible?inactivation?mechanisms?of?PMJ?and?explore?a?potential?application?in?dental?and?other?temperature?sensitive?treatment.?Methods:?In?this?study,?we?employed?a?direct?current,?atmospheric?pressure,?cold?air?PMJ?to?inactivate?bacterias.?Scanning?electron?microscopy?was?employed?to?evaluate?the?morphology?of?S.?aureus?and?showed?rupture?of?cell?walls?after?the?plasma?treatment?and?Optical?emission?spectrum?(OES)?were?used?to?understand?the?possible?inactivation?mechanisms?of?PMJ.?Re-sults:?The?inactivation?rates?could?reach?100%?in?5?min.?When?the?distance?between?the?exit?nozzle?of?the?PMJ?device?and?Petri?dish?was?extended?from?1?cm?to?3?cm,?effective?inactivation?was?also?observed?with?a?similar?inactivation?curve.?Conclusion:?The?inactivation?of?bacteria?is?attributed?to?the?abundant?reactive?oxygen?and?ni-trogen?species,?as?well?as?ultroviolet?radiation?in?the?plasma.?Different?life?spans?and?defensibilities?of?these?killing?agents?may?hold?the?key?to?understanding?the?different?inactivation?curves?at?different?treatment?distances.
- plasma,
- ?Staphylococcus?aureus,
- ?Enterococcus?faecalis,
- ?free?radicals,
- ?reactive?oxygen,
- ?ultroviolet