3.5

CiteScore

2.3

Impact Factor
  • ISSN 1674-8301
  • CN 32-1810/R
Yan Li, Yi Jiang, Yicong Wan, Lin Zhang, Weiwei Tang, Jingjing Ma, Shan Wu, Wenjun Cheng. Medroxyprogestogen enhances apoptosis of SKOV-3 cells via inhibition of the PI3K/Akt signaling pathway[J]. The Journal of Biomedical Research, 2013, 27(1): 43-50. DOI: 10.7555/JBR.27.20120051
Citation: Yan Li, Yi Jiang, Yicong Wan, Lin Zhang, Weiwei Tang, Jingjing Ma, Shan Wu, Wenjun Cheng. Medroxyprogestogen enhances apoptosis of SKOV-3 cells via inhibition of the PI3K/Akt signaling pathway[J]. The Journal of Biomedical Research, 2013, 27(1): 43-50. DOI: 10.7555/JBR.27.20120051

Medroxyprogestogen enhances apoptosis of SKOV-3 cells via inhibition of the PI3K/Akt signaling pathway

  • We sought to assess the effect of progestin on the apoptosis of epithelial ovarian cancer cell line SKOV-3 and via regulation of phosphorylation signaling in. Epithelial ovarian cancer cell line SKOV-3 was treated with me-droxyprogestogen, phosphatidylinositol 3-kinase inhibitor LY294002 and vehicle control. Akt, phospho-Akt, Bcl-2 and phospho-Bad proteins were examined by immunoblotting assays. Medroxyprogestogen-induced apoptosis was assessed by MTT assays and Annexin V apoptosis assay. We found no significant difference in Akt and Bad expression in both the medroxyprogestogen groups and the control group. The levels of phospho-Akt, Bcl-2 and phospho-Bad were decreased in all the medroxyprogestogen groups and significantly decreased in the high dose mitogen-activated protein (MAP) group (10 μmol/L). Viability of SKOV-3 was reduced and apparent apoptosis of SKOV-3 cells was observed with increased doses of MAP. The findings suggest that medroxyprogestogen can induce SKOV-3 cell apoptosis by inhibiting Akt phosphorylation.
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