• ISSN 1674-8301
  • CN 32-1810/R
Volume 35 Issue 3
May  2021
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Shu Wei, Zhang Yanman, Zhang Chen, You Qiang, Zhou Hong, Wen Shuang. Triclosan inhibits the activation of human periodontal ligament fibroblasts induced by lipopolysaccharide from Porphyromonas gingivalis[J]. The Journal of Biomedical Research, 2021, 35(3): 206-215. doi: 10.7555/JBR.34.20200026
Citation: Shu Wei, Zhang Yanman, Zhang Chen, You Qiang, Zhou Hong, Wen Shuang. Triclosan inhibits the activation of human periodontal ligament fibroblasts induced by lipopolysaccharide from Porphyromonas gingivalis[J]. The Journal of Biomedical Research, 2021, 35(3): 206-215. doi: 10.7555/JBR.34.20200026

Triclosan inhibits the activation of human periodontal ligament fibroblasts induced by lipopolysaccharide from Porphyromonas gingivalis

doi: 10.7555/JBR.34.20200026
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  • Corresponding author: ; Shuang Wen and Hong Zhou, Department of Immunology, School of Basic Medical Sciences, Nanjing Medical University, 101 Longmian Avenue, Nanjing, Jiangsu 211166, China. Tel: +86-25-86869456, E-mails: swen@njmu.edu.cn and hongzhou@live.com
  • Received: 2020-02-21
  • Revised: 2020-06-24
  • Accepted: 2020-06-30
  • Published: 2020-08-31
  • Issue Date: 2021-05-28
  • Periodontitis is a highly prevalent, chronic, non-specific, and immunologically devastating disease of periodontal tissues, caused by microbial infection. This study aims to examine the efficacy and protective mechanism of triclosan (TCS), a bisphenolic, non-cationic component of oral care products, against periodontal inflammation induced by lipopolysaccharide purified from Porphyromonas gingivalis (LPS-PG). TCS markedly downregulated interleukin-6 (IL-6), IL-8, and IL-15 in human periodontal ligament fibroblasts (HPDLFs) treated with LPS-PG. By using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach, 318 differentially expressed proteins (161 upregulated and 157 downregulated) were identified in TCS-pretreated HPDLFs. TCS upregulated HSPA5 and HSP90B1 but downregulated HSPA2. Besides, TCS upregulated miR-548i in HPDLFs, which downregulated IL-15. These results indicate that TCS attenuates the activation of HPDLFs and downregulates the inflammatory cytokines through various mechanisms, thus highlighting its protective role in periodontal inflammation.

     

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