Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

Joshua Muli Mutiso; John Chege Macharia; Evans Taracha; Michael Muita Gicheru

doi:10.1016/S1674-8301(12)60002-5

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Article Navigation > The Journal of Biomedical Research > 2012 > 26(1): 8-16

Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5

Citation:

Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5

Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

doi: 10.1016/S1674-8301(12)60002-5

1.
Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya/Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya
2.
Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya
3.
Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

Funds:

This work was supported by a grant from the National Council for Science and Technology, Government of Kenya (No. NCST 51003 CALL2 226)

Received: 23 July 2011
Issue Date: January 2012

Abstract

In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.
- visceral leishmaniasis,
- Leishmania donovani,
- vervet monkey,
- sonicated antigen,
- adjuvants
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)

doi: 10.1016/S1674-8301(12)60002-5

1. Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya/Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

2. Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya

3. Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya

Funds:

This work was supported by a grant from the National Council for Science and Technology, Government of Kenya (No. NCST 51003 CALL2 226)

Received Date: 2011-07-23
Publish Date: 2012-03-05

Key words:

Abstract: In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.

Citation:

Joshua Muli Mutiso, John Chege Macharia, Evans Taracha, Michael Muita Gicheru. Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)[J]. The Journal of Biomedical Research, 2012, 26(1): 8-16. doi: 10.1016/S1674-8301(12)60002-5